SPERMATOGENESIS IN AMPHIBIA AND INSECTS 15 
FAMILY TETTIGONIIDAE 
In contrast to the unusual method of acrosome formation 
found in the grasshoppers, among many of their relatives the 
process would appear to be a very simple one, quite like that in 
the Hemiptera. Thus far I have examined carefully only Ceu- 
thophilus maculatus Harris, but the condition in this form is 
probably similar to that in other crickets and the locustids, 
where the published accounts are very unsatisfactory indeed 
(see Bowen, ’22 a, for a short résumé). In Ceuthophilus I have 
not yet obtained satisfactory Golgi preparations of the sperma- 
tocytes and earliest spermatid stages. However, during the 
early steps in the condensation of the chromophilic substance, 
the acroblast, in the form of a single compact sphere, appears in 
the cytoplasm in its accustomed location in the angle between 
nucleus and nebenkern (fig. 41), indicating an origin from the 
fusion of scattered Golgi elements as in the beetle and the bug. 
The differentiation of the acrosome begins immediately, and 
a small vesicle soon appears in connection with the acroblast 
quite as in the Hemiptera (fig. 42). The vesicle is sometimes 
clear and transparent (fig. 48), but very frequently it takes the 
stain intensely (fig. 42), by which it is rendered more conspicuous. 
I have noticed a similar tendency in Murgantia after certain 
staining technique. The position of the acroblast-acrosome com- 
plex with reference to the nucleus is not constant as in the Hemip- 
tera, and the acrosome often does not touch the nuclear wall 
at all. 
Shortly before the nucleus begins to elongate to form the sperm 
head, the acrosomal vesicle becomes applied to the anterior 
surface of the nucleus (fig. 43), the acroblast still maintaining 
its original attachment to the acrosome. Presently the acro- 
some flattens out on the surface of the nuclear membrane (fig. 
44), and its connection with the acroblast is severed (fig. 45). 
The latter quickly migrates to the base of the head (fig. 46) and 
thence moves backward along the tail exactly as in other insects, 
in all of which its ultimate fate is probably the same. The acro- 
some, already in its definitive position, rounds out to form a 
THE AMERICAN JOURNAL OF ANATOMY, VOL. 30, NO. 1 
