12 ROBERT H. BOWEN 
plasmic elements in the grasshopper seem to be most difficult to 
preserve and stain satisfactorily. My observations thus far are 
based on two species, Rhomaleum micropterum Beauy., and 
Dissosteira carolina Linn. 
The Golgi apparatus in the grasshoppers is present in the 
primary spermatocytes in the form of many scattered Golgi 
bodies not essentially unlike those which I have described in the 
Hemiptera. The division stages have not yet been worked out, 
but at the close of the second maturation division the Golgi 
elements can be demonstrated clearly, being scattered about in 
the cytoplasm around the nucleus and mitochondria (fig. 20). 
The latter consist of a group of rather imperfect threads which 
have been derived from the second maturation division in a man- 
ner somewhat similar to that noted in Hemiptera. These threads 
soon condense to form the typical spherical nebenkern of insects. 
The separate Golgi bodies are not easily demonstrated with 
clearness, but it is certain, especially from later stages, that each 
one is made up of two substances, one of which is more readily 
stained than the other which it partially encloses. (Compare 
with the Hemiptera which I have described fully (Bowen, ’22 a).) 
In one respect, however, the Golgi bodies differ in behavior very 
decidedly from other cases which have been described. ‘They 
remain separate for the most part as distinct bodies (figs. 23 and 
24), and never fuse to form the massive acroblast so character- 
istic of the animal spermatid. Occasionally, it would appear, 
two or three fuse together to form a larger aggregate, but in 
general this does not occur. These Golgi bodies tend now to 
collect near the nuclear membrane, particularly on one side of 
the nebenkern (figs. 21 and 23), and they remain, generally 
speaking, in this vicinity until the nebenkern begins to elongate 
(fig. 24). Then they begin to migrate back along the tail (fig. 
25), and are probably cast out in the protoplasmic mass sloughed 
off the tail at the close of sperm formation. 
In the later spermatid stages and especially just prior to the 
backward migration of the Golgi bodies, an intensely staining glob- 
ule can be made out in contact with the nuclear membrane not 
far from the centriole (figs. 23 and 24). This is at first difficult to 
