ON THE NATURE OF MITOCHONDRIA ONS 
The experiments recorded below were repeated a number of 
times. In some cases the results were not identical in one set 
of experiments. These differences in results were only slight and 
apparently of no particular consequence to the object of the 
experiments. 
The main object in all of the experiments that follow was to 
determine the staining reaction of bacteria after treatment with 
chemicals and heat. 
A. Action of alcohol on bacteria 
Aleohol of various strengths was permitted to act on five 
different strains of bacteria for a period of five hours. 
a. After 95 per cent alcohol. 1. Human tubercle bacilli. Stain 
the same as control, granules appear more distinct than in 
control. 
2. Bovine tubercle bacilli. Stain the same as control, some 
crescent forms apparently not observed in control. 
3. Bacillus megatherium (with spores).. Bacilli stained fainter 
than controls, spores tinted. 
4. Bacillus subtilis. Stained more intensely than control. 
5. Unknown cocci and bacilli from a lymph-node culture, two 
strains of cocci, one intensely stained and the other very faintly 
in controls. The cocci appear to be destroyed. Two strains of 
bacilli (different in length) not observed in controls were intensely 
stained. 
b. After 50 per cent alcohol. 1. Human tubercle bacilli. Some 
bacilli are very faintly stained, others appear to be slightly 
swollen. 
2. Bovine tubercle bacilli. Some indication of disintegration, 
the bacilli intact were decidedly shrunken. 
3. Bacillus megatherium. Bacilli could not be demonstrated 
by staining. The spores were decidedly swollen and in many 
parts of the field they were coalesced (partially dissolved). 
4. Bacillus subtilis. Bacilli could not be demonstrated by 
staining. Field contained intensely stained granular debris. 
5. Unknown cocci and bacilli from lymph-node culture. Field 
full of very minute well-stained cocci (granules?), also a few 
