214. IVAN E. WALLIN 
large well stained cocci. Some of the larger cocci coalesced. 
Few exceedingly small well-stained bacilli. Large bacilli un- 
stained. 
c. After 25 per cent alcohol. 1. Human tubercle bacilli. Bacilli 
stain very faintly and appear shrunken. Granules in bacilli 
not visible. 
2. Bovine tubercle bacilli. Great number of bacilli disin- 
tegrated. 
3. Bacillus megatherium. Bacilli could not be demonstrated 
by staining. Some unstained bacillus-like forms partially coa- 
lesced. Spores coalesced, very few distinct in outline. 
4. Bacillus subtilis. Granular debris, stained. 
5. Unknown cocci and bacilli from lymph-node culture. Cocci 
could not be demonstrated by staining. Few small bacilli 
stained. : 
d. After 10 per cent alcohol. 1. Human tubercle bacilli. Bacilli 
appear more granular than control. Some disintegration. 
2. Bovine tubercle bacilli. Most bacilli are granular, some 
crescent-shaped, some swollen, and some disintegrated. Some 
bacilli intact have a purple color. 
3. Bacillus megatherium. Some unstained swollen bacilli pres- 
ent. Spores coalesced. 
4, Bacillus subtilis. Field contains granular debris which has 
the appearance of minute cocci. 
5. Unkriown cocci and bacilli from lymph-node culture. Few 
intensely stained cocci, bacilli unstained. 
e. After 5 per cent alcohol. 1. Human tubercle bacilli. Only 
a few bacilli intact and stained in thick part of smear, rest of 
field contains debris of disintegration. 
2. Bovine tubercle bacilli. Some disintegration. Appear bet- 
ter preserved than after action of 10 per cent alcohol. 
3. Bacillus megatherium. Bacilli could not be demonstrated 
by staining. Spores completely coalesced. 
4. Bacillus subtilis. Only slight indication of a few unstained 
bacilli. 
5. Unknown cocci and bacilli from lymph-node culture. 
Granular debris that appears like minute cocci. Few minute 
bacilli stained. 
