ON THE NATURE OF MITOCHONDRIA 215 
f. After 2 per cent alcohol. 1. Human tubercle bacilli. AI- 
most completely disintegrated. Few swollen poorly stained 
bacilli present in field. 
2. Bovine tubercle bacilli. Almost completely disintegrated. 
Few swollen poorly stained bacilli present in field. 
3. Bacillus megatherium. Bacilli could not be demonstrated 
by staming. Spores coalesced. 
4, Bacillus subtilis. Few intensely stained fragmented bacilli 
present in field. 
5. Unknown cocci and bacilli from lymph-node culture. This 
preparation appears very much like the control. Bacilli ap- 
parently not stained. | 
B. Action of chloroform and ether on bacteria 
a. After chloroform. 1. Human tubercle bacilli. Bacilli in- 
tact, but appear shrunken and more granular than control. 
2. Bovine tubercle bacilli. Bacilli more faintly stained and 
appear more granular than controls. 
3. Unknown bacilli, culture from intestinal contents of five- 
day-old kitten, two strains of bacilli, large and small. Large 
bacilli more granular than control, smaller forms clear and faintly 
stained. 
4. Bacillus megatherium and spores. Few poorly stained and 
shrunken bacilli present. Spores not visible. 
5. Staphylococcus albus. No normal cocci visible. Remains 
appear like exceedingly minute cocci. 
b. After ether. 1. Human tubercle bacilli. Bacilli could not 
be demonstrated by staining. Remains, granular debris. 
2. Bovine tubercle bacilli. Bacilli could not be demonstrated 
by staining. Remains, granular debris. 
3. Unknown bacilli, culture from intestinal contents a five- 
day kitten. Bacilli could not be demonstrated by staining. 
Granular debris appears like minute cocci. . 
4, Bacillus megatherium and spores. Few disintegrated 
bacilli, remains mostly granular debris. Spores were not visible. 
5. Staphylococcus albus. Remains, granular debris. 
