ON THE NATURE OF MITOCHONDRIA 217 
5. Staphylococcus albus. Cocci unstained and coalesced. 
d. After 5 per cent acetic acid. 1. Human tubercle bacilli. 
Bacilli could not be demonstrated by staming. Remains, minute, 
intensely stained granules. 
2. Bovine tubercle bacilli. (Accidentally destroyed.) 
3. Unknown bacilli, culture from intestinal contents of kitten. 
Bacilli could not be demonstrated by staining. Remains, amor- 
phous and faintly stained. 
4. Bacillus megatherium. Bacilli and spores unstained and 
coalesced. 
5. Staphylococcus albus. Cocci unstained and coalesced. 
D. Action of formaldehyde on bacteria 
Formaldehyde of various strengths (diluted in distilled water) 
was permitted to act on bacteria for a period of six hours. 
a. After 1 per cent formaldehyde. 1. Human tubercle bacilli. 
Bacilli could not be demonstrated by staining. Remains, in- 
tensely stained amorphous masses. 
2. Bovine tubercle bacilli. Poorly stained bacilli that appear 
shrunken. 
3. Unknown bacilli, culture from intestinal contents of kitten. 
Bacilli could not be demonstrated by staining. 
4, Bacillus megatherium. Few unstained and swollen bacilli. 
Spores greatly swollen. 
5. Staphylococcus albus. Cocci faintly stained and swollen. 
b. After 3 per cent formaldehyde. 1. Human tubercle bacilli. 
Bacilli could not be demonstrated by staining. Remains in- 
tensely stained amorphous masses. — 
2. Bovine tubercle bacilli. Bacilli distorted in various ways: 
shrunken, crescent-shaped, and some with large intensely stained 
granules. 
3. Unknown bacilli, culture from intestinal contents of kitten. 
No distinct bacilli stained. Unstained spore-like forms partially 
dissolved. 
4. Bacillus megatherium. Bacilli could not be demonstrated 
by staining. Spores partially dissolved. 
