410 R. S. CUNNINGHAM 
were granules massed between the nuclei, and this mass extended 
arms of dye which surrounded the two nuclei in more or less 
irregular rosettes. In the one case the arrangement suggests 
that the two nuclei are functionally the centers of separate 
cells, while in the other the arrangement implies that the cell is 
truly binucleate and functionally a single unit. The collection 
of granules opposite the long axis of the nucleus was not always 
present, a few cells in every preparation having the principal 
mass at the side of the oval nucleus, but the proportion was 
greatly in favor of the other type. A few granules were often 
seen scattered between the nucleus and the surface or in the 
infranuclear zone, but the principal mass was very seldom in 
either of these two regions. 
In animals stained with carmine the same easier was 
observed with certain modifications. The mass of carmine in 
the end of the cell often extended until it almost filled the entire 
cytoplasmic area, but always the borders were more regular than 
with the trypan blue, the irregular scattered granules being much 
less numerous. The perinuclear rosettes were more infrequent, 
or rather were relatively later in appearing in the case of carmine. 
However, in very heavily stained animals they were to be seen 
in many cells, long fine arms of granules extending irregularly 
across or around the nucleus, or perhaps out into the free areas 
of cytoplasm. A similar condition is shown in figure 7, which 
is from the spleen. It is interesting to recall that the cells of 
the omentum show much more diffusely scattered granules after 
they have been irritated than do the cells of the other areas, 
especially of the spleen (Cunningham, ’22), but the significance 
of this still remains to be studied. 
The spleen 
In the cells covering the spleen the perinuclear rosette de- 
veloped very early in the course of staining with trypan blue, 
but it was usually accompanied by some condensation into the 
type of arrangement which has already been described for the 
general mesothelium. As has been noted, this blue rosette 
