AUTHOR’S ABSTRACT OF THIS PAPER ISSUED 
BY THE BIBLIOGRAPHIC SERVICE, MAY 1 
ON THE NATURE OF MITOCHONDRIA 
III. THE DEMONSTRATION OF MITOCHONDRIA BY BACTERIOLOGICAL 
METHODS 
IV. A COMPARATIVE STUDY OF THE MORPHOGENESIS OF ROOT- 
NODULE BACTERIA AND CHLOROFLASTS 
IVAN E. WALLIN 
Department of Anatomy and the Henry S. Denison Research Laboratories, University 
of Colorado, Boulder 
TWO PLATES (NINE FIGURES) 
III. THE DEMONSTRATION OF MITOCHONDRIA BY BAC- 
TERIOLOGICAL METHODS 
In a former paper (22) the author submitted evidence to 
show that the special mitochondrial technique in general use, 
including the vital janus-green method, is not specific for mito- 
chondria, but will also stain bacteria. While, from a purely 
theoretical consideration, the properties of mitochondria are of 
such a nature that one could hardly expect them to respond to 
bacteriological methods, an analysis of results in this direction is 
not only interesting, but also instructive. 
The author has not been able to find any references in the litera- 
ture to mitochondrial staining with bacteriological methods. 
The results recorded below will serve to indicate not only the 
reactions of mitochondria to such methods, but will also indicate 
possibilities in mitochondrial manipulation. 
MATERIALS AND METHODS 
The tissues used in this study have consisted of various samples 
from young rabbits, kittens, and mature dogs. These tissues 
have included lymph nodes, liver, pancreas, kidney, salivary 
glands, suparenal, thymus, and other tissues. Immediately after 
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