STUDIES ON GERM CELLS ails 
laid eggs of Camponotus herculeanus var. ferrugineus Fabr. 
and called the cleavage nucleus (fig. 41, n). This body is obvi- 
ously not a cleavage nucleus since it is not in the usual position 
occupied by this nucleus, and does not possess the characteristics 
of a cleavage nucleus. Furthermore, it persists during the early 
cleavage stages at the posterior end, whereas the cleavage cells 
(nuclei) are shown by Tanquary in their proper position near the 
anterior pole (fig. 42, cc.).. It seems probable therefore that this 
body belongs to the Class of substances which have been found in 
the eggs of many different kinds of animals and which later become 
part of the material within the primordial germ cells—sub- 
stances to which I have applied the term ‘Keimbahn or germ-line 
determinants.’ This seems all the more probable since it per- 
sists at least until a late cleavage stage (fig. 438, rn.) and later 
there is present a group of cells (fig. 44, kc.) which Tanquary 
describes as a “group of small cells applied to the posterior end 
of the inner peripheral protoplasm,’’ and which further research 
will doubtless prove to be germ cells, No bodies were dis- 
covered in my material that could be recognized as an early stage 
in the formation of the ‘cleavage nucleus’ described and figured 
by Tanquary. 
Discussion. No opportunity was afforded by the material in 
my possession for determining the differentiation of the oocytes 
from the nurse cells, since these two sorts of cells are established 
in ovarioles younger than those in the virgin queens collected 
in the spring. The problem of the separation of germ cells 
(oocytes) from somatic cells (nurse cells) therefore could not be 
solved. The most interesting phenomena exhibited by the 
ovarioles are (1) the presence of the bacteria-like rods and (2) 
the formation and distribution of the secondary nuclei. 
The bacteria-like rods. Blochmann (’84, ’86) was the first 
to observe the bacteria-like rods in the ovarioles of two species 
of ants, Camponotus ligniperda and Formica fusca. Recently 
Tanquary (’13) has observed these bodies in the cytoplasm at the 
posterior end of freshly laid eggs of Camponotus herculeanus 
var. ferrugineus. Blochmann found those in the eggs of Cam- 
ponotus to be from 10 to 12, in length, whereas those in Formica 
