532 ROBERT W. HEGNER 
report (Hegner ’14b) and similar to that described by Martin 
(14) in Agenaspis. 
2. The germ-line-determinant is not the chromatin from an 
oocyte nucleus, as stated in my preliminary paper, but 1t appears 
to be a differentiated part of the protoplasm which arises at an 
early stage (fig. 49) near the posterior end of the oocyte. 
B. Apanteles. 1. The oocyte nucleus has a history similar 
to that described for Copidosoma. Chromosomes are formed 
at an early period, fuse in pairs, become arranged upon an 
asterless spindle (figs. 77-78), and undergo condensation (fig. 79). 
Whether or not they finally form a homogeneous mass could 
not be determined because of the lack of late stages. 
2. Secondary nuclei make their appearance in the almost 
fully grown oocytes. They are distributed throughout the 
anterior half of the oocyte (figs. 75-76), but are entirely absent 
in later stages (fig. 77). Their origin and fate were not 
determined. 
3. The deeply staining substance at the posterior end of the 
older oocytes is probably a germ-line-determinant. It first 
appears in a partially grown oocyte as a dark granular mass, 
which probably represents a differentiated part of the protoplasm 
(fig. 73). Later it passes through the suages described on pages 
527-529 and illustrated in figures 74, 75 and 8&0 to 82. 
C. Gall-flies. 1. The history of the oocyte nucleus of the 
oak-knot gall-fly resembles very closely that of Copidosoma and 
ae ee (figs. 84-87). 
= Nhe oocytes of the blackberry-knot gall- fly contain a chro- 
cain body (figs. 88-91) which probably results from the con- 
densation of chromosomes as in the other forms described above. 
A conspicuous germ-line-determinant is also present near the 
posterior end (figs. 88, 92); the follicle cells divide by mitosis 
(fig. 93). 
3. The half-grown oocytes of the mealy rose gall-fly are pro- 
vided with hundreds of secondary nuclei (fig. 97) which are 
situated in a single layer equidistant from the periphery at all 
points (fig. 96). In younger oocytes (fig. 95) these secondary 
