736 



HUBER. 



EXPLANATION OF PLATE XXXIV. 



All diagrams were made with the aid of the camera lucida. 



Fig. I. Shows longitudinal section of the implanted segment two days after 

 operation, fixed in Flemming's solution, and stained in safranin and licht griin. 

 Leitz y^2 i"-' °i^ immersion, ocular No. 3. The medullary sheath is broken into 

 irregular fragments ; a, nerve corpuscles ; one of which seems to be in the early 

 stages of karyokinetic cell division ; b, polynuclear white blood cell. 



Fig. 2. Cross-section of middle of implanted segment three days after operation, 

 fixed in Flemming's solution. Stained in safranin and licht griin. Leitz J^" oi^ 

 immersion, ocular No. 3. The cut ends of the nerve fibres are of irregular shape, 

 varying in size and degree of degeneration. The myelin is stained black, and the 

 protoplasm of the fibres of a reddish color ; a, a nerve corpuscle ; b, a nerve 

 corpuscle in karyokinetic division. Large number of polynuclear white blood 

 cells in the spaces between the nerve fibres ; c, endoneural connective tissue cells ; 

 d, cell in diaster stage. 



Fig. 3. Longitudinal section of implanted segment five days after operation. 

 Hardened in Miiller's fluid, and stained in anilin blue. Leitz j'o in., oil immersion, 

 ocular No. 3. a, fragments of breaking-down axis cylinder stained deeply blue ; 

 b, nerve corpuscles ; c, polynuclear white blood cell ; d, remains of medullary 

 sheath. 



Fig. 4. Longitudinal section of implanted segment nine days after implantation. 

 Fixed in Flemming's solution, and stained in safranin and licht griin. Leitz yV in., 

 oil immersion, ocular No. 3. Sheaths of implanted fibres filled with d!*faintly 

 granular protoplasm; relatively few nerve corpuscles. The uppermost fibre 

 contains one large and several small fragments of myelin. 



Fig. 5. Degenerating fibre taken from peripheral ulnar one inch below 

 peripheral wound, nine days after cutting the nerve, showing extent of degenera- 

 tion and proliferation of nerve nuclei. Flemming's hardening. Stained in safranin 

 and licht griin. Leitz ^^ in., oil immersion, ocular No. 3. 



Fig. 6. Teased preparation from central wound twenty-one days after implanta- 

 tion. Hardened in Miiller's fluid, and stained in anilin blue and safranin. Leitz 

 Y>i in., oil immersion, ocular No. 3. The figure shows the distal end of a fibre 

 from central ulnar stump, a^ enlarged distal end of axis cylinder ending free in 

 embryonic fibre {c) ; b, fragments of myelin ; d, nerve corpuscles^ 



Fig. 7. Teased preparation from middle of implanted segment twenty-one days 

 after implantation. Hardened in Miiller's fluid, and stained in anilin blue arid 

 safranin after Stroebe. Leitz J^ ^"-i ^"^ immersion, ocular No. 3. Shows the 

 collapsed sheaths of nerve fibres of implanted segment. Polynuclear white blood 

 cells and connective tissue cells between the fibres. 



Fig. 8. Teased preparation from peripheral ulnar twenty-one days after section. 

 Hardened in Miiller's fluid, and stained in anilin blue and safranin. Leitz jV in., 

 oil immersion, ocular No. 3. a, fibre completely degenerated ; b, shows remains 

 of medullary sheath (c); d, ball of myelin containing fragment of old axis cylinder- 



Fig. 9. Cross-section of middle of implanted segment thirty-nine days after 

 implantation. Miiller's fluid hardening. Stroebe's stain. Leitz yV in., oil immer- 

 sion, ocular No. 3. Shows a large amount of connective tissue between old 

 sheaths and new fibres, a, completely degenerated nerve fibres ; b, developing 

 fibres showing axis cylinder surrounded by delicate sheath ; c, new fibre in old 

 sheath. 



