106 GEORGO ORIHAY SHINJI 



can be made out. That they are really a kind of parasitic organ- 

 ism may be proved by the fact, according to Bliichner, that they 

 were successfully raised in culture media by an Italian investi- 

 gator. As I am not familiar with the systematic position of the 

 different kinds of organisms found in the eggs of the several 

 species of coccids, I describe them under the somewhat indefinite 

 yet suggestive terms of 'symbiotic organisms/ 'parasitic organisms' 

 and often as 'polar colony of organisms,' or simply as 'parasites.' 



The manner in which the eggs become infected by the organisms 

 may be observed by examination of older ovarian eggs, such as 

 is shown in figure 25. Here a few dark granular bodies occur in 

 the folHcular epithelial cells situated above the constriction 

 between the nurse cells and egg chamber and also in the egg proper. 

 These dark staining bodies lie at first in the cytoplasm and not 

 in the nucleus of the follicular cells. Since, as illustrated, these 

 parasites are found, not only in the epithelial cells, but also in the 

 lumen between the epithelial layer and nurse cells as well as 

 within the egg, it is clear that they are carried into the egg proto- 

 plasm mostly by the flow of the nurse stream. Soon, however, 

 the chorion is formed around the cytoplasm of the egg, preventing 

 a further immigration of the symbiotic organisms into the egg. 

 In many cases I have noticed the organisms between the ovarian 

 epithelial layer and the chorionic membrane, long after the com- 

 pletion of the chorion around the egg. But no case has been 

 observed where these bodies actually penetrated through the 

 chorion into the egg. 



The condition of the nurse chamber and also that of a portion 

 of a colony of the symbiotic organisms that almost envelops the 

 ovary at this period is shown in figure 40. A careful examination 

 of the specimen brings out the fact that these organisms, so 

 abundant in the cysts adjoining the ovaries, become literally 

 squeezed out of the walls of the cyst into the space between the 

 ovary and the symbiotic organisms. In short, all series of tran- 

 sitional stages between the liberation of the parasites and their 

 entrance into the epithelial cells, and final location at the posterior 

 end of the egg can be found in a few sections of a single female. 



