^68 WHITMAN. [Vol. IV. 



3. Chpsine var. Porte-chaine. 



Moq.-Tand. 1846. PI. xiv. Fig. 5. 

 EBRARD. Nouvelle Monographic des Sangsues Medicinales. Paris, 1857. 



" Aux derniers jours du mois de mai, en 1854, j'ai trouv6 deux glossi- 

 phonies (variete dite Porte-chaine) qui ^taient accouplees. EUes 6taient 

 fix^es, tete a tete, a la face inferieure d'une pierre par leurs ventouses 

 qui ^taient tres-rapproch^es. Leur corps etait contourn6 de telle sorte 

 qu'un de ses cot^s touchait la pierre, et que I'autre etait libre ; elks 

 etaient accolees par la surface abdominale. Une seule de ces ann^lides 

 f^condait I'autre ; car, les ayant separees, je n'apergus qu'une verge. Les 

 ovaires de I'une d'elles se gonfl^rent et se colorerent peu a peu en blanc, 

 et quarante-cijiq jours apres elk fit des ceufs.'^ [pp. 60, 61.] 



Ebrard had the question of reciprocal fecundation in mind, 

 and entirely overlooked the spermatophores. 



4. Clepshie marginata. 

 C. O. WHITMAN. The Embryology of Clepsine. 1878. pp. 8, 9. 



" I have found that eggs taken from the ovary at the time they are 

 about to be laid develop in the normal manner, and have taken advan- 

 tage of this to watch the earliest changes in the ripe egg. I have done 

 this many times, and always with success. I regard this as very strong 

 evidence that impregnation takes place while the eggs are in the ovaries. 

 This is in harmony with the fact that I have found spermatozoa in the 

 ovary two or three days before the time for depositing the eggs. It is 

 barely possible that these spermatozoa found their way into the ovary 

 accidentally during the dissecting. I can only say that no testicular sacs 

 were ruptured during the process ; but the vasa (lefere7itia may have 

 been severed, as they are so minute that one cannot easily see them. 

 The unchanged condition of the gertninal vesicle at the time the eggs have 

 attained their full size renders it probable that fecundation does not take 

 place more thafi four or five days at the longest before the deposit ; but 

 this does not prove that copulation may not have taken place at a much 

 earlier date. / isolated an individual tvhich had Just sucked itself full of 

 blood, and tvhich shoived no signs of eggs through the body-wall, and after 

 fifteen days obtained eggs that developed in the usual manner. Recalling 

 the fact that the growth of the egg from the primary egg-cell requires only 

 twelve to fifteen days, it appears that this specimen was isolated about, 

 or just before, the time when the egg-cell began to grow. In another 

 case eggs were obtained at the end of tivelve days, tvhich developed in the 

 nortnal way. 



