274 JOHNSON. [VoL. IX. 
In order to ascertain, if possible, the real significance of the 
plastogamy in Actinospheerium, I isolated conjugates in watch- 
glasses, and kept a daily record of each culture. For food I 
used a species of Bosmina that was plentiful in the gathering 
containing the Actinospheria, and furnished almost their 
sole diet. They were surprisingly successful in capturing these 
active little crustacea; often two or three would be found 
embedded in the sarcode of one Actinosphzrium. Several of 
the cultures were kept over three months, but none of them 
showed a change in mode of life. The record of one of the 
most successful is given herewith: — 
Pair coalesced, 5 P.M., Jan. 6. 
2 O.3OCACMiat At a7 
2 . pee a Crk kor aed os 
Be ol Me $e (us ced BE is Mabe Ww re Petaeee Wee OF 20 ARMs Pee Ow a0, 
Ge fects Ade) 1) (elute e061 AE) <Ws Tole ae Poe ysik SE OD eae NIE ee gal lis 
3 (2 very large, each formed by coales- 
CENCe GL 2) id xe lay egies o> gle) OS OAM. SO, Iz. 
4 ae ee 0.30. A.M... f) (13" 
4 Gaerenneals IM) SIZE) 23 5. Sah Wtfoieie ye sid 20 CACM, 662 As 
31(2 coalesced) ta. aloat Ba fe) ee OTS Se ui 
5 (one very small), 0%... ts 5 1O-hO A.M, #1 en6, 
5 Pis2OvA. Mag 0 Ts 
Oo japial sey ran beeeser Se UeLape Te biMe, TOs 
5 (one very small) . 1.» » « » » 2 M., sme OT. 
Os sch9 ab 6, a cee RR aR ees Pa Se bP No eee ua 
3 (0 -Pa@ and £-F R41) se Soak, EZ). OM 2s: 
eas Wath Ger neh etek yet, eters 12) °M.; te De 
5 prstnnies “tem fee ie Sent perekte LO, Age te 228; 
@ (and one dead) )3'- 4° 2.45, oi 4-5 4 «| 00.30 AM, “2030. 
6 10.30 A.M., Feb. 1. 
No change in number till Feb. 12. 
4 te Oot 5 Aig oh) 02. 
One jolted Soe aa ace oo) Joel 5 wACMc, “csS> ALI. 
No change in number till Feb. 23. 
247, te eee Seni) Si he A SOA IRE’ Gag. 
one coil 
I (in division), .. ss ie, 2 6). « 1) (3:40 P.M.) S724. 
1 (elongated): = 5 2 4s Si se ep ee SS PUMigy Ooo 256 
I és wi fe! Ge. de S¥ordey deletel Ban EG. tebe Ma eee 20; 
Te(@ormal) 2-550 8 8) de Pe ee NO BO ALN, eer a7; 
I 66 Ge Ue ievhens et Gs Ss, ai LO gu ACM azo: 
