444 Ve (GOR [VoL. IX. 
(c) Limuli that had the entire brain (B Fig. 1, anterior 
oesophageal ganglion) extirpated, are still able to masticate 
and swallow food that is given them. In most other respects 
they are greatly altered. Voluntarily, they never change either 
their place or position, and without some kind of a stimulus 
remain motionless in the most abnormal positions. For in- 
stance, if placed against the side of an aquarium, so that the 
anterior margin of their carapace is down and the spine up; or 
if their sides are down and the spine horizontal, they remain 
so, apparently comfortable. 
A Limulus that has had its brain removed, makes definite 
and appropriate efforts to remove an irritant applied to any 
part. If it fails with one appendage, it makes attempts with 
others. Its movements result from the very slightest stimulus, 
and are orderly and coordinated. 
Limuli that have had part or all of the brain extirpated, no 
longer seek the mates from which they were taken when 
operated. In this respect, I found that they differ from those 
Limuli that had only the olfactory vesicle (sub-frontal sclerite) 
removed. The latter are still able to find their mates. Proving 
that the olfactory vesicle does not aid the male to find the 
female, as it was thought it did (XX). 
(Z7) If we cut between the ganglia of the collar, the animal 
moves toward the injured side, but only until the wound is 
healed. No other change in any of the functions seems to 
have been produced by the operation. 
If, however, one or more ganglia of the collar (C Fig. 1) are 
extirpated, the appendages corresponding to the ganglia become 
paralyzed. Showing that each ganglion controls the activity 
of the corresponding appendage. 
(ec) A Limulus that has the post-oesophageal ganglion 
injured, moves in an awkward manner. The nerves that go to 
the last thoracic appendage, and to the muscles that extend 
abdominal carapace in walking, are injured in the operation. 
Hence it is unable to extend the abdominal part. It is very 
inactive, lying for days in the same spot. Otherwise it does 
not differ from a normal animal. 
