A NEICROSCOPICAL STUDY OF “LHR INERVE, CHIE 
DURING ELECTRICAL STIMULATION: 
C. Fo HODGE, PHD: 
Experiments on spinal and sympathetic ganglion cells of the frog. 
HE purpose of the present series of experiments is to 
observe continuously the process of fatigue in the nerve 
cell. Up to the present series my method has consisted in 
studying the cells at the end of a period of fatigue and only 
after the tissue had passed through the usual processes of 
preparation for the microscope. From the first, it has been 
my desire to study the process continuously in the living cell. 
A report of former work upon the subject of changes in 
ganglion cells, due to electrical stimulation and daily fatigue, 
may be found in a previous number of this Journal.! 
METHOD. 
At first an attempt was made to observe the cells zz sztu 
with circulation disturbed as little as possible. This was done 
by removing the viscera from in front and a portion of the 
spinal column, muscles, etc., from the back, tying all blood 
vessels, thus leaving the pleuroperitoneum with its blood 
vessels and the sympathetic chain intact. Small frogs were 
used, but the large pigment cells in the pleuroperitoneum 
itself, and especially along the blood vessels and sympathetic 
cord, made it impossible to get a clear view of the sympathetic 
ganglion cells. I was obliged to abandon the method. 
It thus became necessary to remove the ganglia from the 
body and free them from their pigmented capsules. In con- 
sequence of this the following method was adopted, and it has 
proved more serviceable than was anticipated. The special 
1 Hodge. A Microscopical Study of Changes Due to Functional Activity in 
Nerve Cells. Journal of Morphology, Vol. 7, p. 95. Boston, 1892. 
449 
