454 HODGE. [Vor LX: 
oe DarTE. 
10.55 
13 Jan. 19 Frog similar to 12—no nuclei or nucleoli visible in any of 
the cells, although they appear clear and free from pigment. 
Stimulation applied, and in 20 minutes faint outline of nuclei 
became visible. After 4 hours and 30 minutes’ stimulation 
faint irregular outline of nuclei visible. — No nucleoli to be seen. 
EOSUEION Or gi NORA SHRINKAGE IN VOLUME oF Nuczeus. 
2.15 0.30 
14 Aug. 14 1ocm. NaClo.65% 46% 
5-00 0.30 1.00 
15 Aug. 14 13 cm. 6“ 24% 42% 
II.05 1.20 
16 Aug. 29 Io cm. Ringer’s solution 48 % 
10.25 0.10 0.20 0.30 0.50 1.20 2.45 
17 Aug. 30 Iocm. “ “ 35% 8% 61% 65% 69% 74% 
9.00 0.23 
18 Aug. 30 E32) COs Mane <c 23% 
2.22 0.10 0.30 0.40 2.45 
19 Aug. 30 F3) cr. | cs 26% 42% 51% 05% 
9-33 0.12 0.35 0.50 1.50 
20 = Aug. 31 130m.) 116 25% 45% 54 54% 
II.10 0.15 0.30 
21 Sept. 2 TQUCIN sce se 22 p37 Yo 
11.45 1.30 
22 Sept. 2 Le gts 0 Ppa 04 48 % 
10.29 0.30 1.00 1.30 2.00 4.44 6.49 
23 Sept. 39 I2cm. “ “18% 29% 43% 43% 69% 75% 
Lxperiments with distal stimulation. 
3-30 o.15 Continuous stimulation. 
24 Aug. 5 13cm. Salt solution 0.65 % 17% 
+ Calcium phosphate 
to saturation. 
5.00 L 0.15 
25 Aug. 13 Io cm. Salt solution 0.65 % 33% Same cell as 24. 
+ Calcium phosphate 
to saturation. 
Lxperiments with central stimulation. 
4.40 0.15 Continuous stimulation. 
26 Aug. 13 1ocm. Salt solution 0.65 % 12% 
+ Calcium phosphate 
to saturation. 
4.45 o.15 Continuous stimulation. 
27 Aig a5 10 cm. & 0% 
o.15 Continuous stimulation. 
28 Aug. 13 1ocm. Salt solution. 30 % Cauelen almost touching elec- 
trode 
9-45 o.15 Continuous stimulation. 
29 Sept. 2 13cm. Ringer’s solution. 0% 
10.30 o.15 Continuous stimulation. 
30 Sept. 2 13 cm. a £ 0% 
Experiments with potassium tartrate solution. 
10.50 0.22 
31 Aug. 28 Iocm. Salt solution 0.65% saturated with calcium phos- 14% 
phate + 0.1 % potassium tartrate. 
Nucleolus moved about in 
nucleus with continuous 
change of shape, and finally 
fragmented and disappeared. 
