No. 3.] SLUDY OF THE NERVES CLILL, 
no signs of movement after a few (less than 
fifteen) seconds’ exposure to the current, and 
speedily disintegrated. Large vorticellae were 
able to endure more; but after two or three 
exposures of fifteen seconds each, they invari- 
ably succumbed. A number of similar tests 
were made with gradually decreasing strengths 
of current, and it was found that with the 
secondary coil at ten, paramoecia, although 
considerably startled, did not appear to be 
injured. Frog’s lucocytes were used in a 
similar way. No relation necessarily exists 
between strength of current injurious to para- 
moecia and to frog’s spinal ganglion cells; 
however, 10 cm. was decided upon as the limit 
of stimulation for remaining experiments. 
Experiments 12 and 13 are anomalous. In 
perfectly clear, slightly pigmented ganglion 
cells no nucleus or nucleolus was to be seen. 
This is not peculiar to the June experiments : 
for even during the winter several cases of the 
kind occurred. They were, at the time, dis- 
carded with the note, “cells not clear, no nuclei 
visible.” The two instances, 12 and 13, are too 
pronounced to leave unrecorded. They force 
upon one comparison with the apparently simi- 
lar disappearance of the germinal vesicle in the 
ovum at maturation. The fact that on stimu- 
lation traces of nuclei, but none of nucleoli 
became visible is also of significance. But 
whatever this may be is at present a matter 
for experiment rather than discussion. The 
subsequent experiments, 14 to 33, made upon 
young frogs, R. temporaria, which had just 
passed the tadpole stage, left nothing to be 
desired by way of clearness of nuclei. 
A fair picture of an experiment can hardly 
be given in a table or by curves. It requires a 
459 
1 
hys.0-99 
0-30 
z: 
1-00 
: (+) 
30 
6-49 
7 
RIGS 4: 
Camera outlines of 
cell, nucleus and nucle- 
olus in Experiment 23. 
(Zeiss, Oc. 6, obj. 40x 
375-) 
Time is given in 
hours and minutes. 
