No. 3.] SLUDY OF THE NERVE CELL. 461 
was impossible also to detect indications of vacuolation in 
the living cell. 
During the whole time nuclei were also subject to closest 
scrutiny in the hope of seeing possibly the movements described 
by Svierczewski,! and of gaining some light as to the function 
of this organ outside of strictly reproductive processes. While 
in normal salt solution or Ringer’s solution, no movement of 
any sort, or only slight changes of shape and position, such as 
are indicated in Fig. 4, could be observed, together with a 
gradual decrease in size. Connected with this latter it was 
possible to make out granules in the nucleolus which moved 
slowly about and in several instances were seen to be extruded 
into the nucleus. Confirmation of the above was immediately 
sought in sections of spinal ganglia already in my possession, 
and was found in abundance in osmic acid specimens stained 
in safranin. The granules here were stained brighter red than 
the body of the nucleolus and several were found partially 
extruded. 
In this connection I may briefly refer to experiments 31 
to 33, although they belong properly to a subsequent paper. 
Potassium tartrate, 0.1%, was added to so- 
dium chloride and calcium phosphate solu- 
tion with a view of giving the cells a trace &. ) 
of potassium and more oxygen, in case there 
was not enough accessible in the plain liquid. 
The somewhat unexpected result may be seen wre 
outlined in Fig. 5. The different stages rep- 
resented in 1-4 were all actually observed and HiGsS> 
camera outlines made in twenty-two minutes. _, C2mr outlines of 
changes in nucleolus in 
The movements of the nucleolus in this case Experiment 31 during 22 
minutes stimulation in 
had “every appearance of beme amoeboid .4 per cent potassiain 
The nucleolus of the control cell changed Sstdded tononmal 
solution. a@-c occurred 
shape somewhat but retained its size, where-  in4 minutes. (Zeiss, Oc. 
3 6, obj. 40375.) 
as the fragments of the stimulated nucleolus eee 
had all dissolved in thirty minutes. Experiments 32 and 33 
1 Svierczewski, Zur Physiologie des Kerns und Kernkoérperchens der Nerven- 
zellen des Sympatheticus. Centralblatt f. d. Med. Wissensch., Berlin, 1860. 
p- 641. 
