462 HODGE. [Ver 1X: 
gave exactly similar results, except that the control nuclei were 
not observed to move. 
A number of the briefer experiments were made with the 
special purpose of testing upon frogs certain results which Vas} 
has recently reported from experiments upon the sympathetic 
ganglion cells of the rabbit. Among these results Vas de- 
scribes a swelling of the cell and especially of the nucleus 
upon continuous stimulation for fifteen minutes, the electrodes 
being applied to the cervical sympathetic 3 cm. below the 
superior cervical ganglion. The nuclei of these cells became 
swollen and clear ; exactly the reverse of my results for spinal 
ganglion cells after a longer period of intermittent stimulation. 
It is of interest to note that this diametrically opposed result 
is obtained by stimulating the nerve in the reverse direction to 
that of the normal passage of the nervous impulse. Hence in 
these experiments stimulation was applied to the dorsal root 
between cord and ganglion. 
Of the seven experiments under this head, four showed no 
change in the appearance of nucleus, two, slight change, 7— 
12%, one, marked change of about 30%. All change observed 
was toward shrinkage, with no tendency to swell. It should 
be added, however, that the present method affords no ade- 
quate test of Vas’s results, since (if stimulation the reverse 
way tends to produce swelling, and if this be anabolic in 
character) no anabolism would be possible in a non-nutrient 
solution. In the two experiments with slight change the 
ganglia were near the electrodes, in the one with marked 
effect it almost touched one electrode, so that the change 
produced was in all probability due to irradiation of stimulus. 
In the present series no attempt has been made to do more 
than to test under new conditions the validity of previous work. 
A method has been devised by which a much easier and more 
complete and satisfactory demonstration of fatigue changes in 
ganglion cells may be made. Former work has found confir- 
mation with the additional result that the effect produced by 
hours’ stimulation of a nerve in the body may be obtained in a 
1 Vas, Ferd, Studien iiber den Bau des Chromatins in der sympathetischen 
Ganglienzelle. Archiv f. Mik. Anat. Bd. 40, S. 375. 1892. 
