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Franklin P. Mall 321 
into sections, by the freezing method, 20 » thick. The specimens are 
to be mounted in glycerine. In the sections of such specimens the 
veins are found filled with blue granules, the pulp with gelatin and 
some blue granules, the terminal arteries with some carmine granules 
and the ampullae with asphalt and some carmine granules. As there 
is no mixing of the fluid gelatin and the asphalt in the pulp, the asphalt 
must take the course of the least resistance from the artery to the vein. 
With Prussian blue this is often directly through Thoma’s Zwischen- 
stiick ; with asphalt the course is always through the pulp-spaces. 
In specimens in which little of the 
asphalt reaches the veins it is found 
that the asphalt passes out into the 
pulp-spaces from the ampullae, then 
piles up, forming clusters like bunches 
of grapes (Text Fig. 1). Each of these 
“orapes” fills a pulp-space. As the 
clusters spread out the globules of as- 
phalt often radiate, leaving interven- 
ing pulp-spaces free. Finally all the 
pulp-spaces are filled with the clusters 
of globules, then they encircle the 
veins and are continued into them as 
fine threads of asphalt. At other times 
some of the veins are surrounded with | Tex? Fia.1, Outline of the terminal 
artery, ampulia and venous sinuses ina 
asphalt globules without many spleen made hemorrhagic by ligating 
Je: 2 thout any of them the vein and then injecting the artery 
communicating wi Tens. _ with asphalt and turpentine. The 
S 8 vith the veins. Some masses of asphalt reach from the artery 
times the inject as _ to the vein and fili the large irregular 
the injection of the asphalt fol Eeacee a thetpulp.s Aantecyaye cine 
lows the reticulum in all directions, 
encircles the veins and enters them at many points. When the 
veins have first been plugged with ultramarine blue the asphalt which 
may enter the veins at points cannot spread far. When fine carmine 
granules are injected with the asphalt but few of them enter the veins, 
most of them lodging in the arteries and. ampullae and some of them 
are scattered throughout the pulp. It appears then that the asphalt 
may push the aqueous fluid from the pulp-spaces and gradually pile 
up as does wax when injected into a lung. Or it may follow the 
reticulum, pushing the watery fluid to the centre of the pulp-spaces, 
as is again the case when the lung into which wax is injected contains 
much air. In this case the lung is filled with many small vesicles of 
wax full of air. The asphalt injections, therefore, give a most decided 
argument in favor of an open circulation. 
