IV Proceedings of the Association of American Anatomists 
tion. An interesting fact confirming the hypothesis of the writer as to 
the aberrant nature of the cardiac glands is the occurrence of parietal 
cells in the cardiac sac of the foetal piy, although none are present in 
the adult. Zymogen granules I found by means of the neutral gentian 
method for the first time in the 21 cm. pig in the cells at the bottoms 
of the rudimentary glands. 
THE NATURE OF THE GRANULE CELLS OF PANETH. Sipney KLEIN 
(Communicated by RoBerT R. BENSLEY). Hull Laboratory of Anatomy, 
University of Chicago. 
The granule cells of Paneth, which are, in the majority of the mam- 
mals in which they have been found, confined to the deep ends of the 
glandulae intestinales of Lieberkiihn, occur in the opossum intermixed 
with goblet cells and cylindrical epithelial cells on the sides of the villi. 
This fact shows clearly that the theory of Bizzozero that the Paneth 
cells are young elements which grow up the sides of the intestinal gland 
and become gradually transformed into typical goblet cells is incorrect. 
The granules of the Paneth cell do not, under any conditions, stain with 
mucicarmine or muchaematein, but, on the contrary, stain intensely in 
iron alum haematoxylin and in the neutral gentian method recommended 
by Bensley for staining zymogen granules, in both of which the mucigen 
granules of the goblet cells remain unstained. Furthermore the 
granules of the Paneth cells give, when tested by MacCallum’s modifica- 
tion of Lilienfeld and Monti’s reaction for phosphorus, a distinct positive 
result. These facts indicate that the Paneth cells are serous cells en- 
gaged in secretion of a product chemically distinct from that of the 
goblet cells, probably an enzyme. 
THE DEVELOPMENT OF THE FRAMEWORK OF THE SUBMAXILLARY 
GLAND. By JosEpH MARSHALL Fiint. Hearst Anatomical Laboratory, 
University of California. AMERICAN JOURNAL OF ANATOMY, Vol. II, No. 1. 
ON THE DIFFERENTIATION OF MUSCULAR TISSUE WHEN REMOVED 
FROM THE INFLUENCE OF THE NERVOUS SYSTEM. By Ross G. 
Harrison. Anatomical Laboratory, Johns Hopkins University. 
The following experiments were made to determine whether a stimu- 
lus from the nervous system (formativer Reiz, Herbst) is necessary to 
initiate the differentiation of striated muscle tissue. 
The medullary cord, the rudiment of the ganglion crest inclusive, 
posterior to the second or third trunk segment, was cut out of frog em- 
bryos (R. sylvatica, 3.7 mm., and R. palustris, 2.9 mm. long) in which 
no histological differentiation in the nervous or muscular systems had 
