324 Granule Cells of Paneth in Intestinal Glands of Mammals 
lamina of which separates it more or less completely from its neighbors. 
The granules stain intensely in iron hematoxylin and in neutral gentian 
but remain quite unaffected by muchaematein or mucicarmine. The 
proximal or basal zone of the cell contains an oval nucleus which is 
surrounded by a small quantity of protoplasm which takes a slightly 
deeper stain than that of neighboring cylindrical cells. Some of the 
cells contain a larger quantity of this basal protoplasm and in a few of 
the cells this exhibits a distinct radial striation, in which case the deeper 
stain is largely confined to the striae. The presence of these basal stria- 
Fic. 3. Bottom of gland of Lieberktihn of the guinea-pig after twenty-four- 
hour fast. From a preparation stained by orange-rubin-toluidene blue 
method. Leitz 1/12, Oc. 4. 
tions was, however, more easily demonstrated in those animals which 
were protected from an excess of physiological stimulation by controlling 
the amount of food taken and supplying it at regular intervals. Fig. 3 
represents the lower end of the gland from the small intestine of a guinea 
pig which had fasted for twenty-four hours after receiving a mixed meal 
of carrots and oats. The aspect of the cells in this case is very different 
from that seen in the animal feeding irregularly. In the first place the 
granules are more than twice as large, and there appears to be a larger 
number, although, for obvious reasons, it is difficult to be sure of this. 
In sections of the intestine stained with toluidene blue alone, or with 
