Sidney Klein 325 
toluidene blue and orange-rubin, the basal cytoplasm of practically every 
cell exhibits a radial striation which is exactly similar to that described 
in various sero-zymogenic gland cells by Bensley, Solger, Garnier and 
others. This character is well illustrated in fig. 3 which is from a speci- 
men stained in the toluidene blue-orange-rubin method. The basal 
filaments stain intensely in toluidene blue, less intensely in iron haema- 
toxylin, but may be observed without difficulty in sections stained with 
alum-haematein. The most effective method of demonstrating the basal 
4 
§ 
Fie. 4. Gland of Lieberktihn of guinea-pig. From preparation treated by 
Macallum’s method for the detection of masked iron; Leitz 1/12, Oc. 4. 
C A yo. 7 icine oa 
ES AE NILDER, 
filaments is by means of the microchemical reaction for organic iron of 
Macallum, because the result is not confused by the faint protoplasmic 
stain which is obtained generally in staining with toluidene blue. This 
method consists in liberating the iron from its organic combinations by 
treatment of sections from material hardened in alcohol with a solution 
of sulphuric acid in alcohol for several hours at 37.5 C. and then 
demonstrating the iron at the point of its liberation by means of haema- 
toxylin (see Macallum, 95). ‘The result, as far as the Paneth cells are 
concerned, is a strong reaction in the substance of the basal filaments and 
in the nuclear chromatin (fig. 4). In some of the Paneth-cells a more 
