Or 
No) 
Robert Bennett Bean 3 
body, which had been injected with carbolic acid in the usual way. These 
brains were suspended in a solution of 40% formalin and 6% sodium 
chloride, vertex up. ; 
In Group 6 the brains were treated in the same way as in Group 5, 
except that they were suspended in 10% formalin and sodium chloride. 
Thirty-eight brains were preserved in this manner. 
Group 7 contains brains that were obtained from frozen and sawed sec- 
tions of cadavers previously injected with formalin. : 
Group 8 contains brains of infants preserved in situ by immersion of 
the head in 10% formalin after opening the membranes so as to allow 
the fiuid to permeate the cerebral structures. 
Group 9 contains brains of Germans from Prof. Waldeyer’s laboratory 
in Berlin. The brains were weighed at the time of removal from the 
body and had been preserved in alcohol several years. 
Perfect preservation of the shape of the brain may be obtained by in- 
jecting the bodies of fresh cadavers with carbolic acid, alcohol, and 
glycerine through the femoral arteries under 120 mm. Hg. pressure, leav- 
ing the body for 12 hours, then after removing the brain, which is firm 
and solid, suspending it in 10% formalin and sodium chloride. All 
brains from No. 1593 onward were suspended base down, thus favoring 
retention of their shape. The first seventy-three brains, up to No. 1659, 
were removed prior to the time at which I began the personal supervision 
of their preservation ; those following were personally attended to and all 
data concerning them is personal. The brain weights given are with the 
dura mater removed, leaving the pia mater and vessels intact. The 
brain weights given in parenthesis are estimated from specimens in which 
the weight, both fresh and after hardening, had been taken. The weight 
of the hardened brain was taken after it had been thoroughly drained. 
The actual weights and areas taken at the time the drainings were 
made are the ones used in the construction of the tables and charts. 
BRAIN OUTLINES. 
Outline drawings are made of the brains in their normal position, 
looking from above; lateral and mesial outlines are drawn after the 
hemispheres have been separated by a sagittal cut through the corpus 
callosum and brain stem. Outlines are also made of the lateral border of 
each hemisphere looking from above, the hemispheres being rotated 
through an angle of 45° on an axis passing beneath the splenium, above 
the anterior commissure, and through the foramen of Munro, This axis 
is drawn in every outline and from it all measurements are made; it is dis- 
