Proceedings of the Association of American Anatomists VII 
Grind the solids thoroughly, dissolve in the warmed alcohol, and filter. 
This is used the same as Leishman’s solution. 
Methylene violet may be prepared by Bernthsen’s method. A fairly 
rapid method of making it is as follows: 
MMC tiem eS, WME reece seh t suas eis eyes 3 5s 5.00 g. 
INSEOOs) GLVSHOIS cite ct oes cie a se 3.00 g. 
PPS tR MCE We eects toes cs cree sie ein, vcr 2 2000.00 ce. 
Dissolve separately, mix and boil for five hours, replacing the water lost 
by evaporation, filter and dry the ppt., which is fairly pure methylene 
violet. Recrystallization (repeated 8 to 10 times) from 95% alcohol puri- 
fies it, vielding beautiful green crystals insoluble in cold water. The 
crude violet may be used for staining, however. ; 
DEVELOPMENT OF MEMBRANOUS LABYRINTH AND ACOUSTIC GAN- 
GLION IN THE HUMAN EMBRYO. By GeorcE L. STREETER. Depart- 
ment of Anatomy, Johns Hopkins University. 
A report supplementing a paper, which was read at the Anatomical 
Congress at Geneva (Verhandl. d. Anatom. Gesell. 1905), and in which 
it was shown by a series of reconstructions that the nerves to the saccule 
and posterior ampulla belong to the vestibular rather than the cochlear 
division of the acoustic nerve. The additional observations included in 
this paper concern features in the development of the ear vesicle, being ad- 
ditional early stages of differentiation, and the correction of certain errors 
in descriptions previously published. It is shown that the saccule instead 
of developing as a pocket from the edge of the cochlea, as described by 
His, Jr., is a portion of the vestibule and is partitioned off from the 
utricle. The saccule is not developed from the cochlea, but the cochlea 
is developed from the saccule, though this process occurs before the 
separation of saccule from utricle is complete. It is further shown that 
each semicircular canal has only one ampulla. 
ON THE EPITHELIAL CELL PROCESSES OF THE SULCUS SPIRALIS 
EXTERNUS. By GeorcE E. SHAmMBAUGH. Hull Laboratory of 
Anatomy, University of Chicago. 
Continuous with the epithelium covering the prominentia spiralis, 
covered over in part, at times, by the cells of Claudius, and lying directly 
in the bottom of the sulcus spiralis externus is a group of epithelial cells 
which possess certain marked peculiarities that distinguish them from 
epthelial cells found elsewhere in the labyrinth. 
In the labyrinth of the pig it was found that these cells first began 
to differentiate from the remainder of the epithelium of the sulcus ex- 
