378 



K. R. BENSLEY 



zymogen is still presout the mitochondria of the affected cells are 

 seen to differ from the surrounding normal cells, in the fact that 

 the rods are shorter and thicker and show a tendency to round up 

 into spheres. As the process advances the mitochondria assumes 

 the condition of large round spherules which gradually diminish 

 in size and ultimately disappear. Meanwhile, all of the zymogen 

 has disappeared from the cell and the number of small granules 

 has so increased that they fill the entire cell. 



m. 



Fig. 15 A pancreatic acinus from the guinea pig, showing cells containing 

 Mankowski granules, and alongside a portion of an islet with A cells and B cells 

 differentially stained; m, Mankowski granules; a, A cells of islet; h, B cells of islet. 

 Hermann's fluid, neutral gentian. 



This change in the pancreas can be easily recognized in the fresh 

 tissue, for the granules have a high refractive index, and so stand 

 out as cloudy cells among the otherwise transparent acinus cells. 

 The granules are larger than those of the islet cells and more re- 

 fractive. 



That these granules are not the same as islet cell granules may 

 be shown in many ways. For example, they do not stain in either 

 neutral red or janus green used intra vitam. Furthermore, they 

 are easily preserved in any of the ordinary fixing fluids and stain 

 readily in gentian violet or safranin, whatever the fixation. It is 



