162 R. KUDO 



appears as a single mass (figs. 41, 42) or more frequently an aggre- 

 gation of three or four deeply staining compact granules (figs. 

 37 to 40). The larger nucleus, which is located near the center 

 of the sporoblast, appears to be vesicular and shows chromatic 

 granules chiefly attached to the network and the nuclear mem- 

 brane (figs. 37 to 40) . The sporoblast, in the meantime, assumes 

 an elongated shape. In the attenuated part, the protoplasm 

 becomes highly vacuolated, and develops into the polar filament 

 (figs. 40 to 49) . In the smear preparation, the sporoblast at this 

 stage exhibits a clear but narrow longitudinal but not straight 

 space (figs. 41 to 43), which becomes broader as the formation of 

 the polar filament progresses, since the polar filament becomes 

 coiled close to the polar capsule which develops at the same time. 

 In sections of young spores, on the other hand, one sees either 

 transverse lines of the coiled filament across the capsule, when 

 they are sectioned near the surface (figs. 47, 50, 51) or dots of 

 uniform size scattered in the capsule when the spore is sectioned 

 through its center (figs. 48, 49). The spore membrane becomes 

 differentiated around the spore, for which no nucleus could be 

 recognized. The sporoplasm occupies rounded portion of the 

 spore. 



As far as the writer is aware, the copulation of isogametes or 

 autogamous union of the two nuclei at the end of schizogony 

 which produces copula or zygote, a sporont, has been reported 

 but by two authors. Mercier ('08 a, '09) observed a caryogamy 

 in the development of Thelohania giardi, which produced a cop- 

 ula, the sporont. Debaisieux ('13) on the other hand, recognized 

 an autogamy at the end of the schizogony of Thelohania varians, 

 which formed a uninucleate zygote or the sporont. The same 

 author ('15) further reported a similar process in Glugea mulleri 

 and Glugea danilewskyi. Perez ('05) mentioned the presence 

 of uninucleate or binucleate Gregarine-like bodies which under- 

 went active movements in Thelohania maenadis, and suggested 

 that these forms might be the phases which belong to the process 

 of fecundation. 



In the present form, the writer has observed forms (figs. 15, 

 18) which apparently correspond with those observed by Perez 



