168 R. KUDO 



The contents are either uniformly granular (fig. 62) or with a 

 vacuole, rounded triangular in form (fig. 63). Thus the appear- 

 ance of fresh spores differs from that of Thelohania magna, 

 Nosema bombycis, N. apis, or N. baetis. The mature spores are 

 4.75 to 6m long and 3 to 4^ broad. 



In fixed preparations, the spore shows quite characteristic 

 appearances. The spore membrane becomes truncate at the end 

 in which the contents are (figs. 65, 67, 68). When stained with 

 Giemsa, the comparatively thick spore membrane remains un- 

 stained, while the contents become differentiated, the cap-shaped 

 part being stained pink red, the other part of the contents assumes 

 a blue color, in which numerous irregular striations are seen (figs. 

 64 to 66, 69) . Near the latter mass there always appears in the 

 pinkish protoplasm one or two spherical granules which stain a 

 deep red (fig. 69) . The pink mass with the deeply staining gran- 

 ules is most probably the sporoplasm, and the bluish colored 

 mass apparently the polar capsule with a coiled polar filament. 

 When the spores are stained with Heidenhain's iron hematoxylin, 

 they assume different aspects according to the degrees of decol- 

 oration. In more or less deeply stained spores, the oval polar 

 capsule is brought into view (fig. 67). In well-differentiated 

 spores, however, one can recognize similar structure found in 

 Giemsa stained spore (fig. 68). 



In Thelohania legeri Hesse, to which the present form is un- 

 doubtedly closely related, a somewhat similar structure was 

 noticed by Hesse ('04). He describes observations upon stained 

 spores as follows: 



L'hematoxyline ferrique colore dans les spores une grosse masse 

 centrale qui represente vraisemblablement I'appareil capsiilaire et le 

 germe. La methode de Romanovsky differencie dans cette masse 

 centrale un amas chromatique ordinairement forme de quatre grains 

 juxtaposes, representant I'element nucleaire primitif qui doit donner 

 naissance ensuite au noyau de la capsule et a ceux du germe, ainsi 

 qu'en temoigne sa division ulterieure, mais c'est un point que je n'ai 

 pas encore suffisamment approfondi. 



Hesse did not state from which end of the spore the filament was 

 extruded, although he says that the anterior end was truncate. 

 It may be understood that the anterior or truncate end is the 



