532 GENCHO FUJIMURA 



especially when lipoid granules are exceedingly plentiful, they 

 entirely disappear. It has already been explained how this lack 

 of plastosomes is not the result of the want of skill in the 

 making of preparations. 



b. As the function begins, lipoid granules first appear con- 

 spicuously, especially at a place which is close to the super- 

 ficial layer, and then vacuoles appear. Sometimes both can 

 appear simultaneously at a comparatively early stage (fig. 9). 



c. The direct relations between the lipoid formation and 

 plastosomes could not be ascertained in my preparations. How- 

 ever, the origin of lipoid granules was found in an extremely 

 small granular body, and on the other hand it is clear that 

 there is a tendency for the plastosomes either to decrease more 

 or less in quantity as function of secretion increases or to en- 

 tirely disappear. 



d. The transparent halos which are sometimes found around 

 the lipoids give the latter an appearance of being the contents 

 of vacuoles, and by reason of such a condition we are led to be- 

 lieve that a certain intimate relation exists between lipoids and 

 the vacuolar formation (figs. 7, 9, and 12). 



e. However, we cannot as yet positively state whether or not 

 all vacuoles without exception have an intimate relation with 

 lipoid granules such as is described above. For instance, I 

 cannot definitely declare whether the formation of the extremely 

 delicate foamy image such as is seen in a part of figures 8, 10, 

 and 12 has been the result of the lipoid granules while in the 

 earliest stage of lipoid formation, that is, while as yet in a stage 

 in which they are exceedingly small, having changed their 

 quality, and caused such small vacuoles to grow in groups, 

 or whether as Mulon quoted before, has observed with respect 

 to the ovarian interstitial cell, at the period of his 'diffused 

 siderophil substance' (though it is still beyond my power to 

 prove that such a period could appear in the syncytium layer), 

 the substance has, instead of forming lipoids directly assumed 

 a vacuolar formation. It is for future investigations to solve 

 such a question. 



