Germ Veils of Leptinotarsa Signaticollis. 179 



that can be readily examined, and that are of interest and import- 

 ance in view of recent developments in this field. 



A polar view of a first spermatocyte spindle at metaphase 

 shows the presence of sixteen chromosomes of various sizes, 

 whose bivalent condition is indicated by a deep groove (Fig. 56) . 

 A longitudinal section of the spindle at anaphase shows at one 

 pole a thick V-shaped body that stains red in safranin-lichtgrtin, 

 preparations exactly like the chromosomes (Fig. 54 x). Of the 

 chromosomes, at the middle of the spindle, there is always one 

 very much larger than the others, consisting of two L-shaped 

 parts placed end to end. In polar views this chromosome can 

 be readily identified by its large size. From the telephase shown 

 in Fig. 57, it is evident that an unequal distribution of chromatin 

 has taken place, one of the daughter cells receiving the odd body 

 in addition to the sixteen chromosomes. This odd body is never 

 found at metaphase with other chromosomes. In Fig. 55 it is 

 shown at x, but in order to see it the focus had to be changed. This 

 figure shows its usual position relative to the other chromosomes, 

 which as a rule it precedes to the pole of the spindle, although it 

 occasionly lags behind them. 



When we come to work out the history of this odd body, it is 

 found to have its origin in the resting stage following the last 

 spermatogonial division, as a deeply basic-staining nucleolus of 

 a bi-partite form (Figs. 48 and 49), which persists throughout the 

 resting period when the ordinary chromosomes are represented 

 by an irregular reticulum. 



Spermatogonial equatorial plates show the presence of two 

 homologous series of chromosomes, the number of which can not 

 be determined satisfactorily at this stage by counting. Following 

 the last spermatogonial division, the cells have the appearance 

 represented in Fig. 45, in which the chromatin is arranged in the 

 form of a split spireme that is closely contracted at one side of 

 the nucleus. Generally at this time, a structure bearing an un- 

 mistakable resemblance to the odd body of the first maturation 

 spindle can be seen ( as in Fig. 39 although this figure represents a 

 young ovocyte) . From this we go to the stage shown in Fig. 46, 

 where the spireme is becoming disentangled. The double nature 



