CHROMOSOMES OF TIGER BEETLES 455 
resemble the small chromosomes of the spermatogonia. When 
viewed from the side, the metaphase chromosomes appear 
exceedingly uniform, but a polar view usually shows one V-shaped 
and a number of irregular chromosomes. Although the present 
material is not especially favorable for a comparison of the 
diploid and haploid chromosomes, a close study of figures 48 
and 50 might suggest that the V’s and U’s of the latter are the 
diploid pairs A and B, respectively. 
The metaphase chromosomes appear on the spindle as biva- 
lents (fig. 52), the elements of which pass irregularly to opposite 
poles (figs. 52 to 55). It is thus difficult to find an anaphase 
plate showing all the chromosomes in one plane. Late ana- 
phases and telophases (figs. 55 to 58) are quite uniform, however, 
in comparison with the drawn-out and massed condition found 
in the first maturation division (figs. 44 and 45). 
8. THE METAMORPHOSIS OF THE SPERMATIDS 
At the close of the second maturation division the telophase 
chromatin mass is transformed into a dark spermatid nucleus, 
containing large granular strands of chromatin (fig. 59). This 
_ heavy network soon shows light areas, indicating a loosening of 
the nuclear content (fig. 60). The chromatin strands then 
become more conspicuous near the nuclear membrane, leaving 
the central part almost clear (figs. 61 and 62). They continue 
to condense until chromatin aggregations are formed, which 
resemble prophase chromosomes of very ‘small cells (fig. 62). 
Whether or not the number of aggregations of chromatin at this 
point represent the haploid number of chromosomes we can 
only surmise. A large chromatin nucleolus is usually very 
prominent throughout these stages. 
- In the early spermatid the nucleus takes a position at one 
side of the irregular cell (figs. 59 to 61). The cytoplasmic part 
of the cell elongates, leaving only a very thin film on one side 
of the nucleus (figs. 60 to 63). In this large mass of cytoplasm 
and near the nuclear wall of the early spermatid can be observed, 
under very favorable conditions, a small area which seems to be 
‘less granular or fibrillar than the remaining cytoplasm. This 
