310 TAKU KOMAI 
MATERIAL AND METHODS 
Squilla oratoria De Haan (S. affinis Berthold) is a stomatopod 
common along the Pacific coast of the milder districts of Japan. 
In the Gulf of Tokyo it is fished in great quantity throughout 
the year and forms a staple food. The material of the present 
study was obtained at intervals from November to May, 1917- 
19. The males were all available for the study, since all of 
them supplied both early and advanced stages of spermato- 
genesis. To secure early stages, however, especially in spring 
months, it was necessary to take the hindmost region of the 
testis. For fixing, various reagents were tried, such as, 1) acetic 
alcohol; 2) acetic sublimate (glacial acetic acid in 3 per cent); 
3) Bouin’s fluid; 4) Worcester’s fluid; 5) Flemming’s strong 
solution, and, 6) Hermann’s fluid. Of these the last two reagents 
were found to give the most satisfactory results and were used 
almost exclusively for the present study. Sections were cut 
from 4 to 10 uw in thickness. Smear preparations were fixed 
either with Bouin’s fluid or with osmic vapor. For staining, 
Heidenhain’s iron-hematoxylin was used, with or without coun- 
terstaining with orange G, Bordeaux red, Congo red, or acid 
fuchsin. Both safranin and Delafield’s hematoxylin were found 
to be less satisfactory than iron-hematoxylin, and they were 
used only to check the above method. 
MALE REPRODUCTIVE ORGAN OF SQUILLA ORATORIA 
Grobben’s description of the male reproductive organ of 
Squilla mantis (’76) applies very well to the same organ of 
Squilla oratoria. As was pointed out by that author, the 
organ is situated directly beneath the heart tube and above the 
digestive tract and hepatic organ, and consists of two distinct 
portions, namely, the gonad proper and the ‘accessory gland.’ 
The former lies in the posterior region of the body, ranging from _ - 
the last thoracic somite to the telson, while the latter is located 
in the middle region, in the exposed thoracic somites. Aside 
from this distinction in situation, the two portions bear close 
resemblance with each other. Both form extremely slender 
