378 MITCHEL CARROLL 
C. Technique and nomenclature 
Individuals 2482, 2503, 2511, 2518, 2525, and 2526 were killed 
with xylol. The killing agent for each animal collected in 1909 
was not recorded. But as only xylol and cyanide were used, it is 
evident, from what has since been learned of the effect of different 
killing agents (McClung, 718), that 954.1 and 980 were killed with 
eyanide and 950 with xylol. In the former two the spindles are 
short, the chromosomes crowded and contracted; in the latter, the 
spindle is long, the chromosomes extended and widely spaced. 
The fixative for all testes was strong Flemming solution. The 
sections on slides 2482 to 2526, inclusive, prepared by the writer, 
were cut either 8 or 10 u in thickness. The slides of individuals 
950, 951, 954.1, and 980 were prepared at the University of 
Kansas, and the sections are 7 uw in thickness. The thin sections 
require more time for study on account of the greater difficulty 
of matching, but are often helpful when examining particular 
chromosomes. 
Flemming’s tricolor and Heidenhain’s iron-hematoxylin were 
used for staining. 
All terms employed in this paper have long been in use by 
students of orthopteran spermatogenesis and are those, in the 
main, proposed by McClung in early papers of this series (Me- 
Clung, ’00, ’05, 714). 
THE TESTES 
The acrididian testis has been described and figured by Sutton 
(00, ’02), Davis (08), and Robertson (’16). In all essential 
features the testis of Camnula agrees with the descriptions and 
illustrations of this organ in other species of the family as given 
by the above authors. But there are certain mathematical 
details in the gross and histological structure of the camnulan 
testis which must be mentioned in order that the probable error 
in the chromosome statistics given beyond can be appreciated. 
The testes are united into a single mass in the manner typical 
of the family. Each testis is composed of a number of cylindrical 
follicles. No mention is made in the literature of the number of 
