INTEGUMENT OF NECTURUS MACULOSUS 501 
the club cells are closely packed, the ordinary cylindrical cells 
of the germinative layer, greatly distorted as if wedged in between 
their large neighbors, assume forms adapted to the space left. 
The nuclei of the club cells are smaller than those of the sur- 
rounding epithelial cells, and are usually spherical, but in many 
instances they become lobed or deeply furrowed. ‘The furrows 
(perhaps more accurately described as constrictions) are some- 
times so pronounced as to give the appearance of a binuclear 
condition which might have arisen by amitosis. The nuclei 
almost always lie near the base of the cells. In several instances 
ovoid cells were observed which, although smaller than the club 
cells and having central nuclei, stained densely and presented 
a general appearance suggesting an early stage of development 
of the club cells. 
The bodies of the club cells color intensely in all plasma stains, 
but in no cases were they found to take any basic stain. Cohn 
(795), however, reports that in axolotl the contents of the Leydig 
cells stain in iron haematoxylin similarly to the secretion of the 
large granular glands. (The affinity of the granules of the 
large glands for this stain, which has been reported by several 
workers, has not been demonstrated in Necturus.) The contents 
vary in appearance, generally looking like a reticulum with a 
very fine granular secretion contained within its meshes. At 
other times the reticular condition does not appear, and the 
cells seem to be crowded with brightly stained secretion granules. 
In one series of sections, however, the reticular and coarse 
granular conditions were found in combination within single 
cells. The granules were larger than any seen elsewhere and 
lay in the meshes of the reticulum. There is some evidence, to 
be advanced later, which indicates that this may be the mature 
condition of the gland cell. 
The cell wall is not always clearly defined, but often appears 
as a mere marginal condensation of the cytoplasm or secretion, 
perhaps due to the action of the fixing reagent. Many writers 
have attached great importance to the appearance of the margin, 
since in this region they hoped to obtain evidence to substantiate 
the idea that these gland cells produced a secretion which escaped 
