316 
condition in the sunfish may be interpreted as a retention of the prim- 
itive but more intricate system. 
Papers Cited. 
Epwarps, H. Mizu, ’58, Lecons sur la physiologie et l’anatomie com- 
parée de Vhomme et des animaux, Tome 3. Paris. 614 pp. 
Parker, G. H., and Davis, F. K., ’99, The Blood Vessels of the Heart 
in Carcharias, Raja, and Amia. 3 Pls. Proceed. Boston Soc. Nat. 
Hist., Vol. 29, No. 8, Oct. 1899, p. 163—178. 
Nachdruck verboten. 
Human Spermatogenesis. 
By E. V. Wırcox, Ph.D., Depart. of Agricult., Washington D.C., U.S.A. 
The material upon which this brief account is based was obtained 
by an operation for hernia upon a man fifty four years of age. Only 
one testis was removed. The hernia was of long standing, having 
existed for thirty two years. There had been no scrotal swelling, 
however, except for one year just previous to the operation. 
The testis was quite normal in respect of size, macroscopic and 
microscopic appearance. There was no inflammatory condition or 
evidence that such had ever been the case and not the slightest trace 
could be seen of atrophy or degeneration in the structure as a whole 
or in any of the cellular elements. 
Immediately after removal the testis was cut into pieces and 
thrown into ZENkEr’s fluid which was prepared as follows: 
Bichromate of potash 2 parts, 
Sulphate of soda 1 part, 
Corrosive sublimate 5 parts, 
Glacial acetic acid 5. parts,” 
Water to make 100 parts. 
The material was allowed to remain in ZenKEr’s fluid for twenty 
four hours. It was then washed in running water for two hours and 
put through the different grades of alcohol, beginning with 50 per cent. 
The material was stained on the slide. It was found that ZENKER’S 
fluid gave excellent results with this material. The pieces of tissue 
were large but the killing fluid had penetrated thoroughly and had 
preserved the most delicate structures. There were no noticeable 
differences in the state of preservation between the inside and outside 
of the pieces. 
