11 



Distribution of Granule Cells in the Regions of the 

 Digestive Tract and in the Mucosa. 



I did not investigate the occurrence of basophile cells in different 

 regions of the tract (through it is probably identical with that of the 

 acidophiles). The material for the study of the basophile cells was 

 taken from a region about three feet above the ileo-cecal valve. 

 These cells have no definite arrangement in the mucosa as do the 

 basophiles of the Carnivora, described by Hardy and Westbrook (3), 

 but are irregularly scattered, occurring more frequently towards the 

 lumen. I have not observed that the basophile cells in the pig wander 

 outside the mucosa. 



For the study of the distribution of acidophiles, preparations were 

 examined from the oesophagus, fundus and pyloric regions of the 

 stomach, small intestine 1 ft., 6 ft., 10 ft. and 20 ft. below pylorus, 



1 ft. and 3 ft. above ileo-cecal valve, colon 6 inches below valve and 



2 ft. above anus. I saw no acidophiles in the oesophagus nor in the 

 fundus of the stomach. A. few occur in the pyloric portion, some of 

 which are similar to those of the intestine, but others are peculiar in 

 that their nuclei appear bright red after Flemming's triple stain. In 

 the small intestine the acidophiles are very numerous. Near the py- 

 lorus there are rarely more than eight in one field (one-twelfth inch 

 objective. No. 4 eye-piece, section 5 micra in thickness), in the jejunum 

 and ileum there are often 30 to 35 in one such field. They seem to 

 be slightly more numerous in the latter region. The colon rarely 

 shows more than six or seven in one such field. 



The acidophiles are more numerous in the depths of the mucosa 

 than in the villi, and differ slightly in character in the two regions. 

 The cells in the villi are usually larger, more polygonal, contain fewer 

 granules and are in closer relation with the reticulum. The acido- 

 philes are not so numerous in the immediate region of the muscularis 

 mucosae. The cells occur occasionally within the latter, also within 

 the submucosa, and occasionally even in the interstitial tissue of the 

 muscular coats. They are not evenly distributed within the mucosa; 

 I noticed in one field (one-twelfth objective, etc. as above) 32 acido- 

 phile cells, in an immediately adjoining field 15. I have seen as many 

 as 38 in one field — section from ileum three feet above valve. Acido- 

 philes do not occur within the lymph nodes of the intestinal wall. 

 I have seen but few examples of their occurrence within the intesti- 

 nal epithelium, one on the villus, others in crypts — observations suf- 

 ficient, however, to establish the fact that acidophiles do wander. Small 



