12 



non-granular wandering cells frequently occur in the epithelium. 

 Unlike Unna's (10) plasma cells , the plasma-mastcells of Krom- 

 PECHER (6), the mastcells of Westpiial (12), the plasma cells of 

 Waldeyer (U), the mastcells of Ehrlich (2), the acidophiles of 

 Bergonzini (1), the acidophiles stand in no relation to blood vessels, 

 chyle vessels, to the surface nor to any lamina. I saw no aggregation 

 of cells which might be centers of proliferation, as described by Hardy 

 and Westbrook (3), from which the cells might migrate. 



Comparison with the Granule Cells of the Blood. 



Granule cells here described do not stand in any relation to blood 

 vessels, hence any relation to the blood cells which they may have 

 cannot be inferred from their position, as in the case of the plasma 

 cells of Krompecher (6). In order to study the granule cells of the 

 blood, smears were allowed to dry in air, then handled in the same 

 way (except the embedding) as were the sections. They were fixed in 

 the ordinary manner, by the fluids used for fixation of the mucosa, 

 washed out, dehydrated, stained and mounted. By this process the 

 instable basophile granules such as occur in the intestinal mucosa 

 would not be expected to be preserved ; staining failed to show them. 

 There are, however, in such preparations finely granular basophile 

 cells which stain metachromatically with methylene blue and thionin. 

 These probably correspond to the finely granular basophiles described 

 by Kanthack and Hardy (5). Being insoluble in water, they cannot 

 be closely related to the basophile granules of the intestinal wall. 



In order to subject the blood cells to the same treatment as 

 those of the mucosa, I dropped blood drawn directly from the animal 

 into boiling absolute alcohol. After some days the cells were pipetted 

 from the alcohol, cleared in xylol, embedded in a Lefevre's embed- 

 ding dish, sectioned, fixed on a slide without water and stained. 

 This method should give the coarsely granular basophiles, if they be 

 in the blood. Staining in 95 % alcoholic solution of methylene blue 

 revealed none however. 



Certain acidophile cells are present in the blood. The coarsely 

 granular acidophiles, the alpha-granules of Ehrlich, are easily demon- 

 strated after fixation either by alcohol or Flemming's mixture. Hae- 

 matoxylin and eosin show granules similar in appearance to those 

 of the acidophile cells in the intestinal mucosa; but they must be dif- 

 ferent in chemical nature from their insolubility in water after fixation 

 by alcohol. Flemming's triple stain on smears of blood which were 

 treated in exactly the same way as were sections of the mucosa, show 



