iiiug them gradually through the difterent grades of alcohol, cleared 

 in chloroform or xylol, and embedded in paraffin. 



The following stains were used : acid-eosin, dissolved in water, in 

 glycerine, and in 95 % alcohol; orange G, dissolved in the same ways; 

 indulin and acid fuchsiu, in solution of glycerine and in anilin e- water ; 

 basic safranin, in aniline-water ; gentian violet, aqueous ; fuchsin, aqueous ; 

 methylene blue, aqueous and in 95 7o alcohol, used at ordinary tempe- 

 ratures and at 56" C; Delafield's haeraatoxylin ; methylene green, 

 aqueous; thionin, in 95% alcohol; polychrome methylene blue; mixture 

 of strong aqueous solution of fuchsin, one part, strong aqueous iodine 

 green, nine parts. Special stains: Flemming's triple (see Lee's Vade- 

 Mecum, 5th edition, p. 213); Heidenhain's iron-haematoxylin ; Biondi- 

 Ehrlich-Heidenhain's tricolor stain ; Ehrlich's triacid mixture ; 

 Ehrlich's mastcell stain ; Ehrlich's neutrophile stain ; Ehrlich's 

 amphophile stain. 



Basophile Granule Cells. 



Within the protoplasm of a few of the cells in the mucosa of the 

 pig's intestine are many basophile granules. They are well preserved 

 only by fixation in absolute alcohol either at room temperature or 

 boiling (one temperature seems as good as the other for the purpose). 

 10% formalin, and corrosive sublimate solution containing 0,6% sodium 

 chloride preserve the granules only imperfectly. After fixation by 

 alcohol the granules are readily soluble in water, hence the stains 

 employed must be used in 95 % alcohol. Methylene blue thus used 

 gives a very clear definition of the granules; its efiectiveness is in- 

 creased by raising the temperature to 56° C. The solubility of 

 the granules in water after fixation by alcohol and the favorable 

 action of methylene blue at higher temperatures are characteristic of 

 basophile granules described by Hardy and Westbrook (3) for the 

 intestinal mucosa of certain other vertebrates. The same points, 

 however, distinguish them from the clasmatocytes of Ranvier (8) 

 which, according to that author, are best fixed by osmic acid^). 

 Dahlia, thionin, and polychrome methylene blue all give imperfect 

 stains of the granules. The other stains used failed to show them. 



The basophile cells are round, oval or spindle-shaped, and often 

 have one or more processes two or three times the length of the cell 

 body. The processes are sometimes branched and pass off into the 



1) Clasmatocytes may also be fixed in 33 1/ 3 % alcohol, or in picric 

 acid, followed by the methyl violet stain. 



