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studied. So far as we could determine, the nerve cells in the 4 

 months starved ganglia were normal and presented all of the usual 

 nerve cell features. 



The appearance of the cells in the 16 months starved was con- 

 fusing for some time. The nucleus seemed normal and was surrounded 

 by a fine granular cytoplasm in which was suspended a golden-yellow 

 oil droplet which gave the fat reaction with Sudan III. We finally 

 were able to dissect out a nearly complete living nerve cell which is 

 shown in photo 5. Here the cell wall is clearly indicated as a sharp 

 line, the nucleus, cytoplasm and oil -droplet are in contact with the 

 cell wall for part of the way 

 but toward the main mass 

 of cells there is indicated a 

 space which is free from 

 granules, etc. This we inter- 

 pret as the effect of the pro- 

 longed starvation, the cavity 

 free from the granules being 

 occupied probably by lymph. 

 Cells fixed and subsequently 

 stained show exactly cor- 

 responding conditions. 



Stomach. In making 

 the histological preparations 

 care was taken to have the 

 conditions uniform through- 

 out. The material here 

 described was all fixed in 

 Bouin's fluid, hardened in 

 alcohol, sectioned in paraffin 

 and stained in iron haemato- 

 xylin and eosin. The sections 

 were uniformly cut at 4 micra. The pieces sectioned were taken from 

 corresponding parts of the various organs. 



When the sections are examined the first striking observation 

 made is that in the specimen starved for the longer period there has 

 been more of shrinkage than in the more fortunate and less starved 

 individual. 



The whole stomach wall is somewhat reduced in thickness, the 

 muscle layers, sub -mucosa and mucosa all being affected. Photo- 

 graphs 6 and 7 indicate the conditions shown in the outer portion of 



Fig. 5. Photograph of an unstained, living 

 nerve cell dissected from the dorsal ganglion of 

 the Necturus starved for 16 months, cw cell 

 wall, Nil nucleus, F fat drop. Vac vacuole. Note 

 the great shrinkage of the cytoplasm away from 

 the cell wall and the presence of a vacuole filled 

 with some sort of lymph which occupies the greater 

 part of the cell. 



