No. 2.] CHANGES IN NERVE CELLS. wj 



the two ganglia to be compared go through every step of the 

 process together. 



Almost every method has been tried in the hope of obtaining 

 some striking reaction. Some such were found, but up to date 

 they have all proved inconstant. Trzebinski (8i) has made a 

 special study of the influence of hardening reagents upon the 

 ganglion cells of the spinal cord. He finds corrosive sublimate 

 one of the best reagents, and states that it does not produce 

 vacuolation of the cell. This method, followed by Gaule's quad- 

 ruple staining, has given the best preparations for the study of 

 granulation and staining (see PL I, Figs. 3-5). Trzebinski, it 

 would seem, did not experiment with osmic acid. This, with 

 haematoxylin and safranin, or all four of the Gaule stains, has 

 given a most perfect preservation of the form of the nucleus and 

 the minute structure of the cell protoplasm. Altmann's methods 

 (2) have been tried a number of times, but although beautiful 

 preparations of gland tissues were obtained, nothing definite was 

 brought out in nerve cells. 



Two widely different animals, the frog and cat, were purposely 

 selected, upon which to experiment. The results which I will 

 now pass to consider are derived from fifteen experiments upon 

 frogs and eleven upon cats. All the experiments will be referred 

 to either singly or in groups. 



Results. 



For sake of brevity little more than a tabulation of the results 

 will be given. For further details, see a former paper (24). 



Frog No. I was given three drops of one per cent curare solu- 

 tion and right sciatic nerve was stimulated continuously for 

 thirty minutes. The three pairs of sciatic ganglia were excised 

 and with those of a control frog hardened in corrosive sublimate. 

 The ninth pair were stained in toto in soda carmine, and for some 

 unaccountable reason scarcely any nucleoli could be found in 

 sections of the stimulated ganglion, while they appeared as usual 

 in the ganglion of the other side and in the control ganglia. A 

 count of the two gave the following : — 



nuclei nucleoli 



Six sections of each contained I .. \ <r \ I'lj 28 



