No. 2.] CHANGES IN NERVE CELLS. 119 



TABLE I. 



Frog No. 7, made reflex. Stimulated two and one-half hours, 

 intervals of rest and stimulation being two minutes. Three 

 sets of one hundred nuclei each. 





Nuclei in ti. Cells in \i. 



mean diameters. mean diameters. 



Resting 13-57 1 39-69 



Stimulated 12.23 \ Set i. 35.00 



Diff. 1.34-' 



Resting 13.94 



^•% \ o^ \^ Stimulated 12.56 [■ Set 2. 



Diff. \.l% 



Resting 14.48 \ 



Stimulated 1 3.26 I Set 3. 



Diff ^ 1.22 J 



Sets I, 2, and 3, volume shrinkage, 24 %.i 



The five succeeding experiments were made with the purpose 

 of getting the greatest possible amount of change ; and under 

 the supposition that this might be obtained, for the frog at 

 least, in shorter time, if the nutrition of the cells was prevented, 

 the frogs were thoroughly bled or the capsules of the ganglia 

 torn off. None of these experiments gave definite results. 

 Sections of both ganglia appear, stained by Gaule's method, 

 redder than normal, indicating apparently a clogging of the 

 cells with decomposition products. Stimulated and resting 

 show alike vacuolation, perhaps the same as that observed 

 by Rosenbach (68) in starving dogs. The nuclei in both are 

 shrunken, but show no marked difference in size. 



Results of a single experiment of this class need be given, 



TABLE n. 



Frog No. 8, bled. Stimulated for seven hours, five minutes 

 of stimulation alternating with five minutes rest. One set of 

 one hundred nuclei. 



Mean diameters 



Ganglia of Sth pair, hardened in 

 corrosive sublimate ar 

 by the Gaule method. 



inglia of Sth pair, hardened m -» ^ . ,r . 1 • 1 



*" . ,,. . J . • 1 Resting 12.36 1 Volume shrinkage, 



corrosive sublimate and stained f ^ • 1 j I 80/ 



bv the Gaule method. J ...... 



1 The volume shrinkage per cent is computed from the mean diameters, treating 

 the nuclei as spheres. 



