356 Journal of Comparative Neurology. 



recognized and the processes followed until they left the plane of 

 the section. The nucleolus appeared as a dark spot within the 

 lighter nucleus ; Nissl bodies could be seen not only in the cell 

 bodies but throughout the entire length of the protoplasmic 

 bridges; fibrilar structures could be discerned both in and around 

 the nucleus and in some instances extending from one cell to 

 another. 



In one case (Fig. 5) two cells were joined in a manner 

 which suggested a degree of incomplete anastomosis. The 

 two cells and the connecting process being in the same plane, 

 the hne of refraction, near the most central cell of the group, 

 (at the point A) could not have been caused by a process entering 

 the cell-body at a higher or lower level or by one which passed 

 over or under the cell. The line of refraction may represent the 

 meeting of the protoplasm of one cell with that of the other 

 without as yet having become homogeneous with it. 



While examining some old Golgi preparations we were 

 pleased to discover a few cases of anastomoses in the cerebral 

 cortex of the cat (Fig. 6). 



In staining with methylene blue the method employed was 

 that used by Fischer, slightly modified to suit the various tis- 

 sues. The method used by us was : — 



(i) Harden in 



10% solution of Formalin 24 hrs. 

 90% Alcohol 24 hrs. 



icx)^ Alcohol 3 or 4 hrs. 



(2) Embed in paraffin. 



Section, 15 to 25 microns in thickness. 



(3) Stain with Griibler's methylene blue solution : 



Frog tissues for 24 hours ; fish, cat and mouse tissues for 

 48. hours. 



(4) Rinse in distilled water, differentiate in anilin oil and 

 alcohol. 



Anilin oil i part. 



Alcohol, go<^c 9 parts. 



