No. 2.] THE DEVELOP AIENT OF THE NEWT 295 



most slightly crenated (Figs. 14, 15, 16). In eggs killed 

 with hot water I have never found such an appearance as 

 Schultze depicts in Fig. 18. With other methods of killing, 

 however, I have sometimes seen a similar pseudopodia-like 

 outline to the germinal vesicle. Since the penetration of any- 

 irritating fluid into an amphibian egg must necessarily be far 

 from rapid, it seems possible that the germinal vesicle may be 

 stimulated to slight amoeboid movements ; so that while in 

 one sense the " pseudopodia " may be an artificial product, in 

 another sense they may be the expression of potential amoeboid 

 capabilities. While I incline, therefore, to the belief that 

 amoeboid movements in the older vesicles are not, in Dieviyc- 

 tybis at least, usual and normal occurrences, I am in full accord 

 w4th Schultze as to the reality of these appearances in young 

 eggs, and can confirm his statement as to the presence of both 

 amoeboid and resting nuclei side by side in the same prepara- 

 tion. It is impossible to set a precise limit to the ceasing of 

 these amoeboid phenomena, but in a general way I can say 

 that it is extremely rare to find germinal vesicles with pseudo- 

 podia after migration of the nucleoli has begun. 



I have never seen the pseudopodia of the vesicle stretch out 

 into the cell and engulf bits of nutrient substance as some 

 observers (Brass) claim to have done, but I nevertheless agree 

 with Korschelt ('89) that the amoeboid movements of the young 

 vesicle are of nutritive significance, and possibly aid in the 

 diffusion of fluid from the nucleus into the cell. In the egfirs 

 of Raua palicstris I have frequently observed the appearance 

 depicted in Fig. 17. The peculiar amoeboid structure at the 

 center of the vesicle with nucleoli clustered around it might 

 almost suggest that the nucleoli had been drawn to the center 

 rather than migrated there independently. I have, however, 

 never observed such an amoeboid center in the germinal 

 vesicle of the newt. 



The curious recticular structure of the germinal vesicle 

 which Iwakawa ('82) has figured in Figs. 19 and 27 is, I am 

 inclined to believe, delusive. I have obtained a similar appear- 

 ance with the use of picro-sulphuric mixture, but not with hot 

 water, corrosive sublimate or Flemming's mixture. One would 



