Benson A. Cohoe Tye} 
discloses the fact that the cells of the dark groups occur invariably in 
close relation to the ductules. Repeated observations, both in fresh and 
hardened sections, fails to show any exception to this rule. 
The granules seen in the dark cells in a fresh section appear to be 
smaller in size than those in the light cells. Considerable variation 
is to be noticed in the size of the individual granules in the dark cells. 
The nuclei in both kinds of cells, in a resting gland, are obscured by 
the great abundance of granules. The action of certain reagents on 
these unfixed granules gives striking evidence of the ready solubility of 
the granules in both kinds of cells. Those found in the clear cells are 
especially soluble in most reagents. With isotonic salt solution the 
granules are not preserved but slowly dissolve. If placed in a one 
per cent solution of acetic acid, the section at once becomes opaque and 
the granules cease to be spherical, although remaining visible, while 
the two cell groups can be no longer distinguished. Glycerine, in con- 
centrated solution, also dissolves the granules. After treatment with 
osmic acid (1 per cent solution), the lumina of the ducts are seen to be 
crowded with dark brown granules, while the granules in the dark cells 
assume a brownish black coloration. When treated with alcohol, or with 
aqueous sublimate, the granules are readily dissolved. Solger, 96, more- 
over, has called attention to their ready solubility in dilute solutions 
of chromic acid, potassium bichromate, acetic acid, and pure water. 
In a thin section (2-3 micra) of a resting gland, fixed in Bensley’s 
fluid, and stained with hematoxylin and eosin, there can be readily 
observed, even with low magnification, in the portion of the gland near 
an intercalated duct, a group of cells loaded with granules, which stain 
intensely with eosin. (Fig. I.) These centrally disposed cells are fol- 
lowed by a second group of cells, the cytoplasm of which stains indiffer- 
ently with eosin and is destitute of granules. At no time does this 
second variety of cell lie in close relation to an intercalated duct, while 
the granule-holding cell, upon repeated observations, is found always 
opening directly into a ductule. After staining with Heidenhain’s iron 
hematoxylin the granules assume a deep blue-black color and are seen 
to be crowded towards the lumen of the tubule. The nuclei, when visible 
among the mass of granules, appear oval in form and are situated towards 
the base of the cell. This proximally disposed group of granular cells 
is of constant occurrence throughout the lobule and has a_ variable 
diameter of from 85-200 micra. The individual cells of the group have 
an average diameter of 24 micra, while the diameter of the nucleus 
averages about 11 micra. 
