Benson A. Cohoe 15) 
cell. Their average diameter is about 28 micra, while that of the 
nucleus is 9 micra. Secretory canaliculi occur abundantly in connection 
with these outer cells, and are seen to ramify between the cells. (Fig. III.) 
The evidence adduced by a study of sections of the normal resting 
gland, stained with certain selective dyes, is most convincing of the 
specificity of these two kind of cells. Sections hardened in Bensley’s 
fluid, and stained with iron hematoxylin and orange G., show the cyto- 
plasm of the granule-holding cells deeply stained by the orange G., while 
the cytoplasm of the clear cells takes on a bluish tint from the hematoxy- 
lin. The granules stain a deep blue-black. With Bensley’s neutral 
gentian method the granules are colored an intense violet. A sharp 
differentiation between the two kinds of cells is afforded by means of 
Mann’s methyl-blue eosin, the granules and cytoplasm, of the granular 
Fig. III. A section through a group of distal alveolar cells, showing secre- 
tion canaliculi. From a preparation stained in iron-alum hematoxylin. 
Leitz Homog. Imm. 1/12. Oc. 4. 
and duct cells staining a deep red, while the cytoplasm of the clear cells 
is colored by the methyl blue. With Nissl’s methylene blue the intra- 
lobular duct and granular cells stain metachromatically, a reddish violet, 
and the clear cells are stained blue. Dahlia (1 per cent aqueous solution) 
also stains the cytoplasm of the granular cells metachromatically; the 
eranules assume a decided reddish color. With this stain the clear cells 
and nuclei are blue, and the cytoplasm of the intralobular duct cells takes 
on a violet color. By means of iron hematoxylin and erythrosin, a 
clear differentiation can be established. The cytoplasm of the granular 
cells stains a deep pink in contrast with the black granules, while the 
cytoplasm of the clear cells is stained a feeble pink by the erythrosin. 
Orcein stains both kinds of cells, the cytoplasm of the intralobular duct 
cells taking up the stain intensely, while the granular cells also stain 
strongly; the cytoplasm of the clear cells displays much less affinity for 
