176 Finer Structure of the Glandula Submavxillaris 
the stain. Unna’s polychrome methylene blue stains the clear cells 
blue and leaves the granular and duct cells with scarcely any coloration. 
In sections stained with hematoxylin and Congo red, the cytoplasm 
of the duct and granular cells is strongly colored by the Congo red, while 
the clear cells take up the blue of the hematoxylin. By means of the 
Ehrlich-Biondi stain, the intralobular duct cells are dyed an orange- 
yellow, the granules and cytoplasm of the granular cells an intense red, 
and the clear cells a violet-blue. This stain was employed by Krause, 95, 
in his study of the glandula submavyillaris of the hedgehog (erinaceus 
europeus) and the results obtained bore a striking similarity to the 
conditions found in the same gland in the rabbit with the use of the 
same stain. With toluidine blue the granular cells and granules stain 
a bluish-green, while the clear cells take on a fairly strong blue color. 
After fixation in Kopsch’s fluid, and staining with a mixture of lichtgriin 
and safranin, the clear cells and all of the nuclei take up the brilliant 
red of the safranin, and the granular cells, as well as the intralobular 
duct cells, assume a violet blue. With Ehrlich’s neutral solution the 
granules stain an intense red, as does also the “rodded” cytoplasm of 
the cells of the intralobular ducts; the cytoplasm of the clear cells stains 
blue. 
By the use of the so-called specific mucin stain of Krause, thionin, 
followed by potassium ferrocyanide, the clear cells stain meta- 
chromatically, giving the red color characteristic for mucous cells. 
The granular and duct cells stain with the thionin and remain unchanged 
after treatment with the potassium ferrocyanide. A similar and sug- 
gestive result may be obtained by staining sections hardened in Kopsch’s 
fluid with Mayer’s muchematein, as modified by Bensley, 03. The clear 
cells when thus treated give a well-marked “ mucous” reaction, while 
the granule and duct cells remain unstained. With other ordinary 
methods of fixation, this so-called mucous reaction cannot be obtained 
in so striking a manner. After formalin fixation, however, the clear 
cells give a feeble reaction with muchexmatein. 
In order to study the relation of the cells of the granular areas to the 
intercalated ducts, a number of Golgi preparations of the gland were 
made. With the resulting impregnation, the granular cell complexes 
appeared as dark brown masses, while the clear cells were stained a light 
yellow. The injected secretion canaliculi were found present in both 
varieties of cells. In the cells of the granular areas the canals appeared 
short, broad and unbranched, a condition which can readily be observed 
in sections of the gland, hardened in aqueous sublimate, and stained with 
