Benson A. Cohoe ; 181 
be differentiated only with difficulty, while all of the cells appeared ex- 
hausted and almost destitute of granules. In the cells of the dark 
areas some few small granules were still present, and in some cells border 
fat granules could be seen. The gland tubules appeared shrunken while 
the nuclei were readily visible and were found to be round and small. 
The hardened and stained specimens of the gland taken from these 
stages revealed conditions which corresponded closely to those of the 
fresh sections. 
The stage of most complete exhaustion was obtained from an animal 
killed nine hours after stimulation. The gland here appeared pale 
LHWILDER” 
Fic. V. A section of an exhausted gland of a rabbit, after a fast of twenty- 
four hours and subsequent stimulation with pilocarpine for a period of nine 
hours. From a preparation stained in iron-alum hematoxylin. Leitz Homog. 
Immo. 1/125 Oc! 4. 
and shrunken. In fresh sections, examined in aqueous humor, the cells 
were found to be free from granules, with the exception of the dark cells 
in which a few still persisted. Atno time did the granules entirely disap- 
pear from these cells, a fact that accords with the observations of Langley, 
78. The few granules remaining in the dark cells were irregular in form 
and of unequal size. The cells bore evidence of shrinkage and the nuclei 
had lost their rotundity. In a fixed and stained specimen of this stage 
(Fig. V), the distinction between the two kinds of cells was less 
characteristic than in the resting gland. The clear cells appeared even 
