182 Finer Structure of the Glandula Submavxillaris 
more shrunken than in the normal gland. The protoplasmic network 
was formed of larger and more irregular meshes, which did not stain 
so readily as in the resting gland. The nucleus was observed to be large 
with no apparent shrinkage, and with nuclear membrane and nucleolus 
well marked. The chromatin, on the other hand, was not so abundant. 
The location of the nucleus was more central in the cell and further 
removed from the basement membrane, than in the resting gland, while 
its form was more spherical with less apparent distortion. 
The cells of the granular areas in the exhausted gland were recognized 
with greater difficulty in stained sections on account of the loss of 
secretion granules through active stimulation. Even here, however, a 
few granules still persisted. Those remaining were smaller than the 
eranules found in a normal gland and were found wholly in the distal 
part of the cell, lying in close proximity to the lumen of the tubule. 
The cytoplasm was diminished in amount and revealed a reticular mesh- 
work. It stained feebly in marked contrast to the strongly staining 
character of the cytoplasm of the resting granular cell. The nuclei 
were located towards the center of the cell and showed little evidence of 
shrinkage. ‘They were observed in this stage, as in the resting gland, 
to be slightly larger than the nuclei of the clear cells, and to be poor in 
chromatin. 
A section of the gland in the exhausted stage, stained with iron hema- 
toxylin, afforded a clear view of the secretion canaliculi. The lumen of 
such a canal, as well as the lumen of a tubule, appeared broader than 
in the resting gland. Ramifications of the canaliculi could plainly be 
observed among the clear cells but were absent from the granular cells. 
The cells of the intercalated and intralobular ducts were somewhat 
shrunken and fallen away from the basement membrane; the proto- 
“rodded ” duct cells were found to stain less 
plasmic striations of the 
intensely than in the resting gland. The blood-vessel in the interlobular 
connective tissue were engorged with red corpuscles. 
The next stage was taken from a gland twelve hours after stimulation, 
when both kinds of cells were found to again contain granules in a fresh 
section. In the cells of the dark areas many granules were present, while 
the clear cells were seen to contain fewer and smaller granules. It 
was, moreover, quite obvious that the dark cell groups occurred in close 
relation to the ductules, in the same position as in the resting gland. 
After a period of twenty hours, the gland, although somewhat shrunken, 
had again assumed in a large degree, the appearance of a normal resting 
