A STUDY OF THE STRUCTURE OF THE GASTRIC GLANDS 
OF THE DOG AND OF THE CHANGES WHICH THEY 
UNDERGO AFTER GASTROENTEROSTOMY AND OCCLU- 
SION OF THE PYLORUS. 
BY 
BASIL C. H. HARVEY, 
Hull Laboratory of Anatomy, University of Chicago. 
WITH 5 FIGURES. 
New methods of fixation and staining introduced by Bensley in his 
work on the alimentary tract, have demonstrated new facts in the minute 
anatomy of this region. ‘They have been especially valuable in their ap- 
plication to the study of the cellular elements of the various digestive 
glands, and he has already apphed them to the study of the stomach in 
many animals. His work was confined, however, to normal anatomy, 
and by this investigation of the structure of the mucous membrane when 
placed under abnormal conditions, and subjected to the action of external 
influences different from those normally acting upon it, it was hoped, 
with the assistance of these improved methods, to learn something which 
might still further advance our knowledge of the nature and relations 
of its highly differentiated cells. 
TECHNIQUE. 
The methods of investigation adopted were as follows. The dogs were 
killed by illuminating gas at various times after operation, always when 
they were in the fasting condition, at least ten hours after feeding. The 
stomach was immediately opened and its condition noted. Strips of 
mucosa at the sites selected for study were then removed. Parallel 
incisions 1 cm. apart were made with a razor through the tunica mucosa, 
so as to isolate strips 1 em. wide and 2 em. long, including 1 em. of 
gastric and 1 cm. of duodenal mucous membrane. These were then 
dissected free from the muscular coat with razor and scissors, and laid 
down in a drop of fixing fluid on a piece of sheet cork with the free 
surface next the cork. It was pinned in place by porcupine quills, and 
AMERICAN JOURNAL OF ANATOMY.—VOL. VI. 
